View source for 3gtk

==Backtracked RNA polymerase II complex with 18mer RNA==
<StructureSection load='3gtk' size='340' side='right' caption='[[3gtk]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3gtk]] is a 13 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GTK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GTK FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3gtg|3gtg]], [[3gtj|3gtj]], [[3gtl|3gtl]], [[3gtm|3gtm]], [[3gto|3gto]], [[3gtp|3gtp]], [[3gtq|3gtq]]</td></tr>
<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gtk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gtk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gtk RCSB], [http://www.ebi.ac.uk/pdbsum/3gtk PDBsum]</span></td></tr>
<table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gt/3gtk_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Transcribing RNA polymerases oscillate between three stable states, two of which, pre- and posttranslocated, were previously subjected to x-ray crystal structure determination. We report here the crystal structure of RNA polymerase II in the third state, the reverse translocated, or "backtracked" state. The defining feature of the backtracked structure is a binding site for the first backtracked nucleotide. This binding site is occupied in case of nucleotide misincorporation in the RNA or damage to the DNA, and is termed the "P" site because it supports proofreading. The predominant mechanism of proofreading is the excision of a dinucleotide in the presence of the elongation factor SII (TFIIS). Structure determination of a cocrystal with TFIIS reveals a rearrangement whereby cleavage of the RNA may take place.

Structural basis of transcription: backtracked RNA polymerase II at 3.4 angstrom resolution.,Wang D, Bushnell DA, Huang X, Westover KD, Levitt M, Kornberg RD Science. 2009 May 29;324(5931):1203-6. PMID:19478184<ref>PMID:19478184</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>

==See Also==
*[[RNA polymerase|RNA polymerase]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: DNA-directed RNA polymerase]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Bushnell, D A.]]
[[Category: Huang, X.]]
[[Category: Kornberg, R D.]]
[[Category: Levitt, M.]]
[[Category: Wang, D.]]
[[Category: Westover, K D.]]
[[Category: Dna binding]]
[[Category: Dna damage]]
[[Category: Dna repair]]
[[Category: Dna-directed rna polymerase]]
[[Category: Isopeptide bond]]
[[Category: Magnesium]]
[[Category: Metal binding]]
[[Category: Nucleotidyltransferase]]
[[Category: Nucleus]]
[[Category: Phosphoprotein]]
[[Category: Transcription]]
[[Category: Transferase]]
[[Category: Transferase-dna-rna hybrid complex]]
[[Category: Transferase/dna-rna hybrid]]
[[Category: Zinc-finger]]