View source for 1f8b

[[Image:1f8b.gif|left|200px]]

 {{Structure
|PDB= 1f8b |SIZE=350|CAPTION= <scene name='initialview01'>1f8b</scene>, resolution 1.8&Aring;
|SITE= 
|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=DAN:2-DEOXY-2,3-DEHYDRO-N-ACETYL-NEURAMINIC ACID'>DAN</scene>
|ACTIVITY= [http://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18] 
|GENE= 
}}

'''NATIVE INFLUENZA VIRUS NEURAMINIDASE IN COMPLEX WITH NEU5AC2EN'''


==Overview==
2,3-didehydro-2-deoxy-N:-acetylneuraminic acid (DANA) is a transition state analog inhibitor of influenza virus neuraminidase (NA). Replacement of the hydroxyl at the C9 position in DANA and 4-amino-DANA with an amine group, with the intention of taking advantage of an increased electrostatic interaction with a conserved acidic group in the active site to improve inhibitor binding, significantly reduces the inhibitor activity of both compounds. The three-dimensional X-ray structure of the complexes of these ligands and NA was obtained to 1.4 A resolution and showed that both ligands bind isosterically to DANA. Analysis of the geometry of the ammonium at the C4 position indicates that Glu119 may be neutral when these ligands bind. A computational analysis of the binding energies indicates that the substitution is successful in increasing the energy of interaction; however, the gains that are made are not sufficient to overcome the energy that is required to desolvate that part of the ligand that comes in contact with the protein.

==About this Structure==
1F8B is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F8B OCA]. 

==Reference==
Analysis of inhibitor binding in influenza virus neuraminidase., Smith BJ, Colman PM, Von Itzstein M, Danylec B, Varghese JN, Protein Sci. 2001 Apr;10(4):689-96. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11274459 11274459]
[[Category: Exo-alpha-sialidase]]
[[Category: Influenza a virus]]
[[Category: Single protein]]
[[Category: Colman, P M.]]
[[Category: Danylec, B.]]
[[Category: Itzstein, M Von.]]
[[Category: Smith, B J.]]
[[Category: Varghese, J N.]]
[[Category: CA]]
[[Category: DAN]]
[[Category: NAG]]
[[Category: dana]]
[[Category: glycosylated protein]]
[[Category: hydrolase]]
[[Category: influenza protein]]
[[Category: neuraminidase]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:05:49 2008''