View source for 1elq

[[Image:1elq.gif|left|200px]]

 {{Structure
|PDB= 1elq |SIZE=350|CAPTION= <scene name='initialview01'>1elq</scene>, resolution 1.80&Aring;
|SITE= 
|LIGAND= <scene name='pdbligand=K:POTASSIUM+ION'>K</scene> and <scene name='pdbligand=PLP:PYRIDOXAL-5'-PHOSPHATE'>PLP</scene>
|ACTIVITY= 
|GENE= 
}}

'''CRYSTAL STRUCTURE OF THE CYSTINE C-S LYASE C-DES'''


==Overview==
FeS clusters are versatile cofactors of a variety of proteins, but the mechanisms of their biosynthesis are still unknown. The cystine C-S lyase from Synechocystis has been identified as a participant in ferredoxin FeS cluster formation. Herein, we report on the crystal structure of the lyase and of a complex with the reaction products of cystine cleavage at 1.8- and 1.55-A resolution, respectively. The sulfur-containing product was unequivocally identified as cysteine persulfide. The reactive persulfide group is fixed by a hydrogen bond to His-114 in the center of a hydrophobic pocket and is thereby shielded from the solvent. Binding and stabilization of the cysteine persulfide represent an alternative to the generation of a protein-bound persulfide by NifS-like proteins and point to the general importance of persulfidic compounds for FeS cluster assembly.

==About this Structure==
1ELQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Synechocystis_sp. Synechocystis sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ELQ OCA]. 

==Reference==
Crystal structure of the cystine C-S lyase from Synechocystis: stabilization of cysteine persulfide for FeS cluster biosynthesis., Clausen T, Kaiser JT, Steegborn C, Huber R, Kessler D, Proc Natl Acad Sci U S A. 2000 Apr 11;97(8):3856-61. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10760256 10760256]
[[Category: Single protein]]
[[Category: Synechocystis sp.]]
[[Category: Clausen, T.]]
[[Category: Huber, R.]]
[[Category: Kaiser, J T.]]
[[Category: Kessler, D.]]
[[Category: Steegborn, C.]]
[[Category: K]]
[[Category: PLP]]
[[Category: fes cluster biosynthesis]]
[[Category: nif]]
[[Category: pyridoxal 5'-phosphate]]
[[Category: thiocysteine]]

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