View source for 3zy7

==Crystal structure of computationally redesigned gamma-adaptin appendage domain forming a symmetric homodimer==
<StructureSection load='3zy7' size='340' side='right' caption='[[3zy7]], [[Resolution|resolution]] 1.09&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3zy7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZY7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZY7 FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene><br>
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2a7b|2a7b]], [[1gyu|1gyu]], [[1gyw|1gyw]], [[1w63|1w63]], [[1gyv|1gyv]]</td></tr>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zy7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zy7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zy7 RCSB], [http://www.ebi.ac.uk/pdbsum/3zy7 PDBsum]</span></td></tr>
<table>
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== Publication Abstract from PubMed ==
Computational design of novel protein-protein interfaces is a test of our understanding of protein interactions and has the potential to allow modification of cellular physiology. Methods for designing high-affinity interactions that adopt a predetermined binding mode have proved elusive, suggesting the need for new strategies that simplify the design process. A solvent-exposed backbone on a beta-strand is thought of as "sticky" and beta-strand pairing stabilizes many naturally occurring protein complexes. Here, we computationally redesign a monomeric protein to form a symmetric homodimer by pairing exposed beta-strands to form an intermolecular beta-sheet. A crystal structure of the designed complex closely matches the computational model (rmsd = 1.0 A). This work demonstrates that beta-strand pairing can be used to computationally design new interactions with high accuracy.

Computational design of a symmetric homodimer using beta-strand assembly.,Stranges PB, Machius M, Miley MJ, Tripathy A, Kuhlman B Proc Natl Acad Sci U S A. 2011 Dec 20;108(51):20562-7. Epub 2011 Dec 5. PMID:22143762<ref>PMID:22143762</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>

==See Also==
*[[Adaptin|Adaptin]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Mus musculus]]
[[Category: Kuhlman, B.]]
[[Category: Machius, M.]]
[[Category: Miley, M J.]]
[[Category: Stranges, P B.]]
[[Category: Tripathy, A.]]
[[Category: Computational design]]
[[Category: Endocytosis]]
[[Category: Protein design]]