View source for 1b7o

[[Image:1b7o.gif|left|200px]]

 {{Structure
|PDB= 1b7o |SIZE=350|CAPTION= <scene name='initialview01'>1b7o</scene>, resolution 1.8&Aring;
|SITE= 
|LIGAND= <scene name='pdbligand=NA:SODIUM ION'>NA</scene>
|ACTIVITY= [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] 
|GENE= 
}}

'''VERIFICATION OF SPMP USING MUTANT HUMAN LYSOZYMES'''


==Overview==
The stability profile of mutant protein (SPMP) (Ota,M., Kanaya,S. and Nishikawa,K., 1995, J. Mol. Biol., 248, 733-738) estimates the changes in conformational stability due to single amino acid substitutions using a pseudo-energy potential developed for evaluating structure-sequence compatibility in the structure prediction method, the 3D-1D compatibility evaluation. Nine mutant human lysozymes expected to significantly increase in stability from SPMP were constructed, in order to experimentally verify the reliability of SPMP. The thermodynamic parameters for denaturation and crystal structures of these mutant proteins were determined. One mutant protein was stabilized as expected, compared with the wild-type protein. However, the others were not stabilized even though the structural changes were subtle, indicating that SPMP overestimates the increase in stability or underestimates negative effects due to substitution. The stability changes in the other mutant human lysozymes previously reported were also analyzed by SPMP. The correlation of the stability changes between the experiment and prediction depended on the types of substitution: there were some correlations for proline mutants and cavity-creating mutants, but no correlation for mutants related to side-chain hydrogen bonds. The present results may indicate some additional factors that should be considered in the calculation of SPMP, suggesting that SPMP can be refined further.

==Disease==
Known diseases associated with this structure: Amyloidosis, renal OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=153450 153450]], Microphthalmia, syndromic 1 OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=309800 309800]]

==About this Structure==
1B7O is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B7O OCA]. 

==Reference==
Experimental verification of the 'stability profile of mutant protein' (SPMP) data using mutant human lysozymes., Takano K, Ota M, Ogasahara K, Yamagata Y, Nishikawa K, Yutani K, Protein Eng. 1999 Aug;12(8):663-72. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10469827 10469827]
[[Category: Homo sapiens]]
[[Category: Lysozyme]]
[[Category: Single protein]]
[[Category: Nishikawa, K.]]
[[Category: Ogasahara, K.]]
[[Category: Ota, M.]]
[[Category: Takano, K.]]
[[Category: Yamagata, Y.]]
[[Category: Yutani, K.]]
[[Category: NA]]
[[Category: human lysozyme]]
[[Category: mutant stability]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:07:21 2008''