View source for 1aks

[[Image:1aks.jpg|left|200px]]

 {{Structure
|PDB= 1aks |SIZE=350|CAPTION= <scene name='initialview01'>1aks</scene>, resolution 1.8&Aring;
|SITE= 
|LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene>
|ACTIVITY= [http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] 
|GENE= 
}}

'''CRYSTAL STRUCTURE OF THE FIRST ACTIVE AUTOLYSATE FORM OF THE PORCINE ALPHA TRYPSIN'''


==Overview==
The first crystal structure of an active autolysate form of porcine alpha-trypsin (APT), a two-chain molecule obtained from the limited autolysis of porcine beta-trypsin at position Lys145-Ser146, has been determined. APT crystallizes in space group P2(1)2(1)2(1) with one protein molecule in the asymmetric unit. The structure was solved by molecular replacement followed by refinement using X-PLOR to an R factor of 0.200 and an R(free) of 0.285 for 8.0-1.8 A data with r.m.s deviations from ideal values of 0.01 A and 1.7 degrees for bond lengths and bond angles, respectively. Comparison with inactive autolysate porcine epsilon-trypsin (EPT) and porcine beta-trypsin in complex with bittergourd trypsin inhibitor (MCT) revealed a small but systematic directional chain shift around the active-site residues from APT to EPT to MCT.

==About this Structure==
1AKS is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AKS OCA]. 

==Reference==
The first structure at 1.8 A resolution of an active autolysate form of porcine alpha-trysoin., Johnson A, Krishnaswamy S, Sundaram PV, Pattabhi V, Acta Crystallogr D Biol Crystallogr. 1997 May 1;53(Pt 3):311-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15299934 15299934]
[[Category: Protein complex]]
[[Category: Sus scrofa]]
[[Category: Trypsin]]
[[Category: Johnson, A.]]
[[Category: Krishnaswamy, S.]]
[[Category: Pattabhi, V.]]
[[Category: Sundaram, P V.]]
[[Category: CA]]
[[Category: hydrolase]]
[[Category: serine protease]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:58:56 2008''