View source for 3bff

[[Image:3bff.jpg|left|200px]]<br /><applet load="3bff" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="3bff, resolution 1.9&Aring;" />
'''class A beta-lactamase SED-G238C complexed with faropenem'''<br />

==Overview==
SED-1, a class A beta-lactamase from Citrobacter sedlakii, is a CTX-M-type extended-spectrum beta-lactamase that has the ability to hydrolyze expanded-spectrum cephalosporins such as cefotaxime. SED-1 and a SED mutant in which Gly238 has been replaced by a cysteine, forming a disulfide bridge with the other Cys residue located at position 69 (SED-G238C), have been crystallized. The crystals belong to the monoclinic space group C2, with unit-cell parameters a = 188.09, b = 73.65, c = 105.41 A, beta = 121.67 degrees for SED-1 and a = 187.64, b = 73.2, c = 103.89 A, beta = 121.89 degrees for the SED-G238C mutant. X-ray diffraction data were collected to maximum resolutions of 2.4 A for SED-1 and 2.0 A for SED-G238C.

==About this Structure==
3BFF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Citrobacter_sedlakii Citrobacter sedlakii] with <scene name='pdbligand=SCN:'>SCN</scene>, <scene name='pdbligand=FPM:'>FPM</scene> and <scene name='pdbligand=SFR:'>SFR</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BFF OCA]. 

==Reference==
Crystallization and preliminary X-ray diffraction study of the class A beta-lactamase SED-1 and its mutant SED-G238C from Citrobacter sedlakii., Petrella S, Pernot L, Sougakoff W, Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):125-8. Epub 2003, Dec 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14684905 14684905]
[[Category: Beta-lactamase]]
[[Category: Citrobacter sedlakii]]
[[Category: Single protein]]
[[Category: Pernot, L.]]
[[Category: Petrella, S.]]
[[Category: Sougakoff, W.]]
[[Category: FPM]]
[[Category: SCN]]
[[Category: SFR]]
[[Category: acyl-enzyme]]
[[Category: beta-lactamase]]
[[Category: class a sed-g238c]]
[[Category: faropenem]]
[[Category: hydrolase]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:05:20 2008''