View source for 2r83

[[Image:2r83.jpg|left|200px]]<br /><applet load="2r83" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="2r83, resolution 2.70&Aring;" />
'''Crystal structure analysis of human synaptotagmin 1 C2A-C2B'''<br />

==Overview==
Release of neurotransmitter from synaptic vesicles requires the Ca2+/phospholipid-binding protein synaptotagmin 1. There is considerable evidence that cooperation between the tandem C2 domains of synaptotagmin is a requirement of regulated exocytosis; however, high-resolution structural evidence for this interaction has been lacking. The 2.7 A crystal structure of the cytosolic domains of human synaptotagmin 1 in the absence of Ca2+ reveals a novel closed conformation of the protein. The shared interface between C2A and C2B is stabilized by a network of interactions between residues on the C-terminal alpha-helix of the C2B domain and residues on loops 1-3 of the Ca2+-binding region of C2A. These interactions alter the overall shape of the Ca2+-binding pocket of C2A, but not that of C2B. Thus, synaptotagmin 1 C2A-C2B may utilize a novel regulatory mechanism whereby one C2 domain could regulate the other until an appropriate triggering event decouples them.

==About this Structure==
2R83 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=AC1:Cl+Binding+Site+For+Residue+A+101'>AC1</scene>, <scene name='pdbsite=AC2:Cl+Binding+Site+For+Residue+A+102'>AC2</scene>, <scene name='pdbsite=AC3:Cl+Binding+Site+For+Residue+A+103'>AC3</scene>, <scene name='pdbsite=AC4:Cl+Binding+Site+For+Residue+A+104'>AC4</scene>, <scene name='pdbsite=AC5:Cl+Binding+Site+For+Residue+A+105'>AC5</scene>, <scene name='pdbsite=AC6:Cl+Binding+Site+For+Residue+B+106'>AC6</scene>, <scene name='pdbsite=AC7:Cl+Binding+Site+For+Residue+B+107'>AC7</scene> and <scene name='pdbsite=AC8:Cl+Binding+Site+For+Residue+A+108'>AC8</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R83 OCA]. 

==Reference==
Structure of human synaptotagmin 1 C2AB in the absence of Ca2+ reveals a novel domain association., Fuson KL, Montes M, Robert JJ, Sutton RB, Biochemistry. 2007 Nov 13;46(45):13041-8. Epub 2007 Oct 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17956130 17956130]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Fuson, K L]]
[[Category: Montes, M.]]
[[Category: Robert, J J]]
[[Category: Sutton, R B.]]
[[Category: CL]]
[[Category: c2a-c2b]]
[[Category: calcium]]
[[Category: cell junction]]
[[Category: cytoplasmic vesicle]]
[[Category: endocytosis]]
[[Category: endocytosis/exocytosis complex]]
[[Category: exocytosis]]
[[Category: glycoprotein]]
[[Category: lipoprotein]]
[[Category: membrane]]
[[Category: metal-binding]]
[[Category: palmitate]]
[[Category: phosphorylation]]
[[Category: synapse]]
[[Category: transmembrane]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:45:18 2008''