View source for 2qxi

[[Image:2qxi.jpg|left|200px]]<br /><applet load="2qxi" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="2qxi, resolution 1.000&Aring;" />
'''High resolution structure of Human Kallikrein 7 in Complex with Suc-Ala-Ala-Pro-Phe-chloromethylketone'''<br />

==Overview==
hK7 or human stratum corneum chymotryptic enzyme belongs to the human tissue kallikrein (hKs) serine proteinase family and is strongly expressed in the upper layers of the epidermis. It participates in skin desquamation but is also implicated in diverse skin diseases and is a potential biomarker of ovarian cancer. We have solved x-ray structures of recombinant active hK7 at medium and atomic resolution in the presence of the inhibitors succinyl-Ala-Ala-Pro-Phe-chloromethyl ketone and Ala-Ala-Phe-chloromethyl ketone. The most distinguishing features of hK7 are the short 70-80 loop and the unique S1 pocket, which prefers P1 Tyr residues, as shown by kinetic data. Similar to several other kallikreins, the enzyme activity is inhibited by Zn(2+) and Cu(2+) at low micromolar concentrations. Biochemical analyses of the mutants H99A and H41F confirm that only the metal-binding site at His(99) close to the catalytic triad accounts for the noncompetitive Zn(2+) inhibition type. Additionally, hK7 exhibits large positively charged surface patches, representing putative exosites for prime side substrate recognition.

==About this Structure==
2QXI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=K7J:'>K7J</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Stratum_corneum_chymotryptic_enzyme Stratum corneum chymotryptic enzyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.117 3.4.21.117] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QXI OCA]. 

==Reference==
Chymotryptic specificity determinants in the 1.0 A structure of the zinc-inhibited human tissue kallikrein 7., Debela M, Hess P, Magdolen V, Schechter NM, Steiner T, Huber R, Bode W, Goettig P, Proc Natl Acad Sci U S A. 2007 Oct 9;104(41):16086-91. Epub 2007 Oct 1. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17909180 17909180]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Stratum corneum chymotryptic enzyme]]
[[Category: Bode, W.]]
[[Category: Debela, M.]]
[[Category: Goettig, P]]
[[Category: Hess, P.]]
[[Category: Magdolen, V.]]
[[Category: Schechter, N M.]]
[[Category: K7J]]
[[Category: alternate conformations]]
[[Category: alternative splicing]]
[[Category: chloromethyl ketone]]
[[Category: glycoprotein]]
[[Category: hydrolase]]
[[Category: protease]]
[[Category: s1 pocket]]
[[Category: secreted]]
[[Category: serine protease]]
[[Category: zymogen]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:43:05 2008''