View source for 2pei

[[Image:2pei.jpg|left|200px]]<br /><applet load="2pei" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="2pei, resolution 2.700&Aring;" />
'''Crystal structure of selenomethionine-labeled RbcX'''<br />

==Overview==
After folding, many proteins must assemble into oligomeric complexes to become biologically active. Here we describe the role of RbcX as an assembly chaperone of ribulose-bisphosphate carboxylase/oxygenase (Rubisco), the enzyme responsible for the fixation of atmospheric carbon dioxide. In cyanobacteria and plants, Rubisco is an approximately 520 kDa complex composed of eight large subunits (RbcL) and eight small subunits (RbcS). We found that cyanobacterial RbcX functions downstream of chaperonin-mediated RbcL folding in promoting the formation of RbcL(8) core complexes. Structural analysis revealed that the 15 kDa RbcX forms a homodimer with two cooperating RbcL-binding regions. A central cleft specifically binds the exposed C-terminal peptide of RbcL subunits, enabling a peripheral surface of RbcX to mediate RbcL(8) assembly. Due to the dynamic nature of these interactions, RbcX is readily displaced from RbcL(8) complexes by RbcS, producing the active enzyme. The strategies employed by RbcX in achieving substrate specificity and efficient product release may be generally relevant in assisted assembly reactions.

==About this Structure==
2PEI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Synechococcus_elongatus_pcc_7942 Synechococcus elongatus pcc 7942]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PEI OCA]. 

==Reference==
Structure and function of RbcX, an assembly chaperone for hexadecameric Rubisco., Saschenbrecker S, Bracher A, Rao KV, Rao BV, Hartl FU, Hayer-Hartl M, Cell. 2007 Jun 15;129(6):1189-200. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17574029 17574029]
[[Category: Single protein]]
[[Category: Synechococcus elongatus pcc 7942]]
[[Category: Bracher, A.]]
[[Category: Hartl, F U.]]
[[Category: Hayer-Hartl, M.]]
[[Category: Rao, B Vasudeva.]]
[[Category: Rao, K Vasudeva.]]
[[Category: Saschenbrecker, S.]]
[[Category: chaperone]]
[[Category: helix bundle]]
[[Category: protein complex assembly]]

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