View source for 2pc5

[[Image:2pc5.jpg|left|200px]]<br /><applet load="2pc5" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="2pc5, resolution 2.20&Aring;" />
'''Native crystal structure analysis on Arabidopsis dUTPase'''<br />

==Overview==
The deoxyuridine triphosphate nucleotidohydrolase gene from Arabidopsis thaliana was expressed and the gene product was purified. Crystallization was performed by the hanging-drop vapour-diffusion method at 298 K using 2 M ammonium sulfate as the precipitant. X-ray diffraction data were collected to 2.2 A resolution using Cu K alpha radiation. The crystal belongs to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 69.90, b = 70.86 A, c = 75.55 A. Assuming the presence of a trimer in the asymmetric unit, the solvent content was 30%, with a V(M) of 1.8 A3 Da(-1).

==About this Structure==
2PC5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/dUTP_diphosphatase dUTP diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.23 3.6.1.23] Known structural/functional Site: <scene name='pdbsite=AC1:Mg+Binding+Site+For+Residue+A+167'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PC5 OCA]. 

==Reference==
Purification, crystallization and preliminary crystallographic analysis of deoxyuridine triphosphate nucleotidohydrolase from Arabidopsis thaliana., Bajaj M, Moriyama H, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1;63(Pt, 5):409-11. Epub 2007 Apr 14. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17565183 17565183]
[[Category: Arabidopsis thaliana]]
[[Category: Single protein]]
[[Category: dUTP diphosphatase]]
[[Category: Bajaj, M.]]
[[Category: Moriyama, H.]]
[[Category: MG]]
[[Category: arabidopsis]]
[[Category: dutpase]]
[[Category: hydrolase]]
[[Category: magnesium]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:28:07 2008''