View source for 2ol3

[[Image:2ol3.gif|left|200px]]<br /><applet load="2ol3" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="2ol3, resolution 2.90&Aring;" />
'''crystal structure of BM3.3 ScFV TCR in complex with PBM8-H-2KBM8 MHC class I molecule'''<br />

==Overview==
Binding degeneracy is thought to constitute a fundamental property of the T-cell antigen receptor (TCR), yet its structural basis is poorly understood. We determined the crystal structure of a complex involving the BM3.3 TCR and a peptide (pBM8) bound to the H-2K(bm8) major histocompatibility complex (MHC) molecule, and compared it with the structures of the BM3.3 TCR bound to H-2K(b) molecules loaded with two peptides that had a minimal level of primary sequence identity with pBM8. Our findings provide a refined structural view of the basis of BM3.3 TCR cross-reactivity and a structural explanation for the long-standing paradox that a TCR antigen-binding site can be both specific and degenerate. We also measured the thermodynamic features and biological penalties that incurred during cross-recognition. Our data illustrate the difficulty for a given TCR in adapting to distinct peptide-MHC surfaces while still maintaining affinities that result in functional in vivo responses. Therefore, when induction of protective effector T cells is used as the ultimate criteria for adaptive immunity, TCRs are probably much less degenerate than initially assumed.

==About this Structure==
2OL3 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=NAG:'>NAG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OL3 OCA]. 

==Reference==
How much can a T-cell antigen receptor adapt to structurally distinct antigenic peptides?, Mazza C, Auphan-Anezin N, Gregoire C, Guimezanes A, Kellenberger C, Roussel A, Kearney A, van der Merwe PA, Schmitt-Verhulst AM, Malissen B, EMBO J. 2007 Apr 4;26(7):1972-83. Epub 2007 Mar 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17363906 17363906]
[[Category: Mus musculus]]
[[Category: Protein complex]]
[[Category: Auphan-Anezin, N.]]
[[Category: Gregoire, C.]]
[[Category: Guimezanes, A.]]
[[Category: Kearney, A.]]
[[Category: Kellenberger, C.]]
[[Category: Malissen, B.]]
[[Category: Mazza, C.]]
[[Category: Merwe, P A.Van der.]]
[[Category: Roussel, A.]]
[[Category: Schmitt-Verhulst, A M.]]
[[Category: NAG]]
[[Category: class i mhc]]
[[Category: h-2kbm8]]
[[Category: t cell receptor]]
[[Category: tcr-pmhc complex]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:19:43 2008''