View source for 2o1t

[[Image:2o1t.jpg|left|200px]]<br /><applet load="2o1t" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="2o1t, resolution 3.20&Aring;" />
'''Structure of Middle plus C-terminal domains (M+C) of GRP94'''<br />

==Overview==
GRP94, an essential endoplasmic reticulum chaperone, is required for the conformational maturation of proteins destined for cell-surface display or export. The extent to which GRP94 and its cytosolic paralog, Hsp90, share a common mechanism remains controversial. GRP94 has not been shown conclusively to hydrolyze ATP or bind cochaperones, and both activities, by contrast, result in conformational changes and N-terminal dimerization in Hsp90 that are critical for its function. Here, we report the 2.4 A crystal structure of mammalian GRP94 in complex with AMPPNP and ADP. The chaperone is conformationally insensitive to the identity of the bound nucleotide, adopting a "twisted V" conformation that precludes N-terminal domain dimerization. We also present conclusive evidence that GRP94 possesses ATPase activity. Our observations provide a structural explanation for GRP94's observed rate of ATP hydrolysis and suggest a model for the role of ATP binding and hydrolysis in the GRP94 chaperone cycle.

==About this Structure==
2O1T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O1T OCA]. 

==Reference==
Structures of GRP94-nucleotide complexes reveal mechanistic differences between the hsp90 chaperones., Dollins DE, Warren JJ, Immormino RM, Gewirth DT, Mol Cell. 2007 Oct 12;28(1):41-56. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17936703 17936703]
[[Category: Canis lupus familiaris]]
[[Category: Single protein]]
[[Category: Dollins, D E.]]
[[Category: Gewirth, D T.]]
[[Category: Immormino, R M.]]
[[Category: Warren, J J.]]
[[Category: chaperone]]
[[Category: endoplasmin]]
[[Category: gp96]]
[[Category: grp94]]
[[Category: hsp82]]
[[Category: hsp90]]
[[Category: htpg]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:13:29 2008''