View source for 2nww

[[Image:2nww.gif|left|200px]]<br /><applet load="2nww" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="2nww, resolution 3.2&Aring;" />
'''Crystal structure of GltPh in complex with TBOA'''<br />

==Overview==
Secondary transporters are integral membrane proteins that catalyse the movement of substrate molecules across the lipid bilayer by coupling substrate transport to one or more ion gradients, thereby providing a mechanism for the concentrative uptake of substrates. Here we describe crystallographic and thermodynamic studies of Glt(Ph), a sodium (Na+)-coupled aspartate transporter, defining sites for aspartate, two sodium ions and d,l-threo-beta-benzyloxyaspartate, an inhibitor. We further show that helical hairpin 2 is the extracellular gate that controls access of substrate and ions to the internal binding sites. At least two sodium ions bind in close proximity to the substrate and these sodium-binding sites, together with the sodium-binding sites in another sodium-coupled transporter, LeuT, define an unwound alpha-helix as the central element of the ion-binding motif, a motif well suited to the binding of sodium and to participation in conformational changes that accompany ion binding and unbinding during the transport cycle.

==About this Structure==
2NWW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii] with <scene name='pdbligand=TB1:'>TB1</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NWW OCA]. 

==Reference==
Coupling substrate and ion binding to extracellular gate of a sodium-dependent aspartate transporter., Boudker O, Ryan RM, Yernool D, Shimamoto K, Gouaux E, Nature. 2007 Jan 25;445(7126):387-93. Epub 2007 Jan 17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17230192 17230192]
[[Category: Pyrococcus horikoshii]]
[[Category: Single protein]]
[[Category: Boudker, O.]]
[[Category: Gouaux, E.]]
[[Category: Ryan, R.]]
[[Category: Shimamoto, K.]]
[[Category: Yernool, D.]]
[[Category: TB1]]
[[Category: amino acid transporter]]
[[Category: aspartate transporter]]
[[Category: binding site]]
[[Category: inhibitor binding binding]]
[[Category: transmembrane transporter]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:11:56 2008''