View source for 2jv3

[[Image:2jv3.gif|left|200px]]<br /><applet load="2jv3" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="2jv3" />
'''Ets-1 PNT domain (29-138) NMR structure ensemble'''<br />

==Overview==
The Pointed (PNT) domain and an adjacent mitogen-activated protein (MAP) kinase phosphorylation site are defined by sequence conservation among a subset of ets transcription factors and are implicated in two regulatory strategies, protein interactions and posttranslational modifications, respectively. By using NMR, we have determined the structure of a 110-residue fragment of murine Ets-1 that includes the PNT domain and MAP kinase site. The Ets-1 PNT domain forms a monomeric five-helix bundle. The architecture is distinct from that of any known DNA- or protein-binding module, including the helix-loop-helix fold proposed for the PNT domain of the ets protein TEL. The MAP kinase site is in a highly flexible region of both the unphosphorylated and phosphorylated forms of the Ets-1 fragment. Phosphorylation alters neither the structure nor monomeric state of the PNT domain. These results suggest that the Ets-1 PNT domain functions in heterotypic protein interactions and support the possibility that target recognition is coupled to structuring of the MAP kinase site.

==About this Structure==
2JV3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. This structure supersedes the now removed PDB entry 1BQV. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JV3 OCA]. 

==Reference==
Structure of the Ets-1 pointed domain and mitogen-activated protein kinase phosphorylation site., Slupsky CM, Gentile LN, Donaldson LW, Mackereth CD, Seidel JJ, Graves BJ, McIntosh LP, Proc Natl Acad Sci U S A. 1998 Oct 13;95(21):12129-34. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9770451 9770451]
[[Category: Mus musculus]]
[[Category: Single protein]]
[[Category: Kang, H.]]
[[Category: Lawrence, M P.]]
[[Category: Lee, G M.]]
[[Category: Schaerpf, M.]]
[[Category: Slupsky, C M.]]
[[Category: alpha-helical bundle]]
[[Category: dna-binding]]
[[Category: ets-1 pointed (pnt) domain]]
[[Category: map kinase phosphorylation site]]
[[Category: nucleus]]
[[Category: transcription factor]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:06:08 2008''