View source for 1v14

[[Image:1v14.gif|left|200px]]<br />
<applet load="1v14" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1v14, resolution 2.90&Aring;" />
'''CRYSTAL STRUCTURE OF THE COLICIN E9, MUTANT HIS103ALA, IN COMPLEX WITH MG+2 AND DSDNA (RESOLUTION 2.9A)'''<br />

==Overview==
Controversy surrounds the metal-dependent mechanism of H-N-H, endonucleases, enzymes involved in a variety of biological functions, including intron homing and DNA repair. To address this issue we, determined the crystal structures for complexes of the H-N-H motif, containing bacterial toxin colicin E9 with Zn(2+), Zn(2+).DNA, and, Mg(2+).DNA. The structures show that the rigid V-shaped architecture of, the active site does not undergo any major conformational changes on, binding to the minor groove of DNA and that the same interactions are made, to the nucleic acid regardless of which metal ion is bound to the enzyme., The scissile phosphate contacts the single metal ion of the motif through, distortion of the DNA brought about by the insertion of the Arg-96-Glu-100, salt bridge into the ... [[http://ispc.weizmann.ac.il/pmbin/getpm?15190054 (full description)]]

==About this Structure==
1V14 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with MG as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Deoxyribonuclease_I Deoxyribonuclease I]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.1 3.1.21.1]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1V14 OCA]]. 

==Reference==
Structure-based analysis of the metal-dependent mechanism of H-N-H endonucleases., Mate MJ, Kleanthous C, J Biol Chem. 2004 Aug 13;279(33):34763-9. Epub 2004 Jun 8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15190054 15190054]
[[Category: Deoxyribonuclease I]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Kleanthous, C.]]
[[Category: Mate, M.J.]]
[[Category: MG]]
[[Category: beta-beta-alpha metal motif]]
[[Category: colicin]]
[[Category: hnh motif]]
[[Category: homing endonucleases]]
[[Category: hydrolase]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 16:18:03 2007''