View source for 2gv5

[[Image:2gv5.gif|left|200px]]<br /><applet load="2gv5" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="2gv5, resolution 3.00&Aring;" />
'''crystal structure of Sfi1p/Cdc31p complex'''<br />

==Overview==
Centrins are calmodulin-like proteins present in centrosomes and yeast spindle pole bodies (SPBs) and have essential functions in their duplication. The Saccharomyces cerevisiae centrin, Cdc31p, binds Sfi1p on multiple conserved repeats; both proteins localize to the SPB half-bridge, where the new SPB is assembled. The crystal structures of Sfi1p-centrin complexes containing several repeats show Sfi1p as an alpha helix with centrins wrapped around each repeat and similar centrin-centrin contacts between each repeat. Electron microscopy (EM) shadowing of an Sfi1p-centrin complex with 15 Sfi1 repeats and 15 centrins bound showed filaments 60 nm long, compatible with all the Sfi1 repeats as a continuous alpha helix. Immuno-EM localization of the Sfi1p N and C termini showed Sfi1p-centrin filaments spanning the length of the half-bridge with the Sfi1p N terminus at the SPB. This suggests a model for SPB duplication where the half-bridge doubles in length by association of the Sfi1p C termini, thereby providing a new Sfi1p N terminus to initiate SPB assembly.

==About this Structure==
2GV5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GV5 OCA]. 

==Reference==
Structural role of Sfi1p-centrin filaments in budding yeast spindle pole body duplication., Li S, Sandercock AM, Conduit P, Robinson CV, Williams RL, Kilmartin JV, J Cell Biol. 2006 Jun 19;173(6):867-77. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16785321 16785321]
[[Category: Protein complex]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Conduit, P T.]]
[[Category: Kilmartin, J V.]]
[[Category: Li, S.]]
[[Category: Robinson, C V.]]
[[Category: Sandercock, A M.]]
[[Category: Williams, R L.]]
[[Category: cdc31p]]
[[Category: centrin]]
[[Category: centrosome]]
[[Category: sfi1p]]
[[Category: spindle pole body]]

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