View source for 2fpk

[[Image:2fpk.jpg|left|200px]]<br /><applet load="2fpk" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="2fpk, resolution 2.10&Aring;" />
'''RadA recombinase in complex with ADP'''<br />

==Overview==
Members of a superfamily of RecA-like recombinases facilitate a central strand exchange reaction in the DNA repair process. Archaeal RadA and Rad51 and eukaryal Rad51 and meiosis-specific DMC1 form a closely related group of recombinases distinct from bacterial RecA. Nevertheless, all such recombinases share a conserved core domain which carries the ATPase site and putative DNA-binding sites. Here we present the crystal structure of an archaeal RadA from Methanococcus voltae (MvRadA) in complex with ADP and Mg2+ at 2.1 A resolution. The crystallized RadA-ADP filament has an extended helical pitch similar to those of previously determined structures in the presence of nonhydrolyzable ATP analogue AMP-PNP. Structural comparison reveals two recurrent conformations with an extensive allosteric effect spanning the ATPase site and the putative DNA-binding L2 region. Varied conformations of the L2 region also imply a dynamic nature of recombinase-bound DNA.

==About this Structure==
2FPK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanococcus_voltae Methanococcus voltae] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=K:'>K</scene> and <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. This structure supersedes the now removed PDB entry 1Z4B. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FPK OCA]. 

==Reference==
Crystal structure of Methanococcus voltae RadA in complex with ADP: hydrolysis-induced conformational change., Qian X, Wu Y, He Y, Luo Y, Biochemistry. 2005 Oct 25;44(42):13753-61. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16229465 16229465]
[[Category: Methanococcus voltae]]
[[Category: Single protein]]
[[Category: He, Y.]]
[[Category: Luo, Y.]]
[[Category: Moya, I A.]]
[[Category: Qian, X.]]
[[Category: Wu, Y.]]
[[Category: ADP]]
[[Category: K]]
[[Category: MG]]
[[Category: atpase]]
[[Category: co-factors]]
[[Category: potassium-dependence]]
[[Category: protein-atp complex]]
[[Category: rada/adp complex]]

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