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[[Image:2fav.gif|left|200px]]<br /><applet load="2fav" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="2fav, resolution 1.800&Aring;" />
'''Crystal structure of SARS macro domain in complex with ADP-ribose at 1.8 A resolution'''<br />

==Overview==
Macro domains constitute a protein module family found associated with specific histones and proteins involved in chromatin metabolism. In addition, a small number of animal RNA viruses, such as corona- and toroviruses, alphaviruses, and hepatitis E virus, encode macro domains for which, however, structural and functional information is extremely limited. Here, we characterized the macro domains from hepatitis E virus, Semliki Forest virus, and severe acute respiratory syndrome coronavirus (SARS-CoV). The crystal structure of the SARS-CoV macro domain was determined at 1.8-Angstroms resolution in complex with ADP-ribose. Information derived from structural, mutational, and sequence analyses suggests a close phylogenetic and, most probably, functional relationship between viral and cellular macro domain homologs. The data revealed that viral macro domains have relatively poor ADP-ribose 1"-phosphohydrolase activities (which were previously proposed to be their biologically relevant function) but bind efficiently free and poly(ADP-ribose) polymerase 1-bound poly(ADP-ribose) in vitro. Collectively, these results suggest to further evaluate the role of viral macro domains in host response to viral infection.

==About this Structure==
2FAV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_sars_coronavirus Human sars coronavirus] with <scene name='pdbligand=APR:'>APR</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FAV OCA]. 

==Reference==
Structural and functional basis for ADP-ribose and poly(ADP-ribose) binding by viral macro domains., Egloff MP, Malet H, Putics A, Heinonen M, Dutartre H, Frangeul A, Gruez A, Campanacci V, Cambillau C, Ziebuhr J, Ahola T, Canard B, J Virol. 2006 Sep;80(17):8493-502. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16912299 16912299]
[[Category: Human sars coronavirus]]
[[Category: Single protein]]
[[Category: Egloff, M P.]]
[[Category: MSGP, Marseilles Structural Genomics Program.@.AFMB.]]
[[Category: Malet, H.]]
[[Category: APR]]
[[Category: marseilles structural genomics program @ afmb]]
[[Category: msgp]]
[[Category: protein-adp-ribose complex]]
[[Category: structural genomics]]
[[Category: structural proteomics in europe]]

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