View source for 1qno

[[Image:1qno.gif|left|200px]]<br />
<applet load="1qno" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1qno, resolution 2.00&Aring;" />
'''THE 3-D STRUCTURE OF A TRICHODERMA REESEI B-MANNANASE FROM GLYCOSIDE HYDROLASE FAMILY 5'''<br />

==Overview==
The crystal structure of the catalytic core domain of beta-mannanase from, the fungus Trichoderma reesei has been determined at a resolution of 1.5, A. The structure was solved using the anomalous scattering from a single, non-isomorphous platinum complex with two heavy-metal sites in space group, P2(1). The map computed with the experimental phases was enhanced by the, application of an automated model building and refinement procedure using, the amplitudes and experimental phases as observations. This approach is, expected to be of more general application. The structure of the native, enzyme and complexes with Tris-HCl and mannobiose are also reported: the, mannobiose binds in subsites +1 and +2. The structure is briefly compared, with that of the homologous beta-mannanase from the ... [[http://ispc.weizmann.ac.il/pmbin/getpm?10666621 (full description)]]

==About this Structure==
1QNO is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Hypocrea_jecorina Hypocrea jecorina]] with NAG and TRS as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Mannan_endo-1,4-beta-mannosidase Mannan endo-1,4-beta-mannosidase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.78 3.2.1.78]]. Structure known Active Site: MAN. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QNO OCA]]. 

==Reference==
The three-dimensional structure of a Trichoderma reesei beta-mannanase from glycoside hydrolase family 5., Sabini E, Schubert H, Murshudov G, Wilson KS, Siika-Aho M, Penttila M, Acta Crystallogr D Biol Crystallogr. 2000 Jan;56(Pt 1):3-13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10666621 10666621]
[[Category: Hypocrea jecorina]]
[[Category: Mannan endo-1,4-beta-mannosidase]]
[[Category: Single protein]]
[[Category: Murshudov, G.]]
[[Category: Penttila, M.]]
[[Category: Sabini, E.]]
[[Category: Schubert, H.]]
[[Category: Siika-Aho, M.]]
[[Category: Wilson, K.S.]]
[[Category: NAG]]
[[Category: TRS]]
[[Category: anomalous scattering]]
[[Category: mannanase]]
[[Category: trichoderma reesei]]

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