View source for 2eyn

[[Image:2eyn.gif|left|200px]]<br /><applet load="2eyn" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="2eyn, resolution 1.800&Aring;" />
'''Crystal structure of the actin-binding domain of human alpha-actinin 1 at 1.8 Angstrom resolution'''<br />

==Overview==
Alpha-actinin belongs to the spectrin family of actin crosslinking and bundling proteins that function as key regulators of cell motility, morphology and adhesion. The actin-binding domain (ABD) of these proteins consists of two consecutive calponin homology (CH) domains. Electron microscopy studies on ABDs appear to support two competing actin-binding models, extended and compact, whereas the crystal structures typically display a compact conformation. We have determined the 1.7A resolution structure of the ABD of alpha-actinin 1, a ubiquitously expressed isoform. The structure displays the classical compact conformation. We evaluated the two binding models by surface conservation analysis. The results show a conserved surface that spans both domains and corresponds to two previously identified actin-binding sites (ABS2 and ABS3). A third, and probably less important site, ABS1, is mostly buried in the compact conformation. However, a thorough examination of existing structures suggests a weak and semi-polar binding interface between the two CHs, leaving open the possibility of domain reorientation or opening. Our results are consistent with a two-step binding mechanism in which the ABD interacts first in the compact form observed in the structures, and then transitions toward a higher affinity state, possibly through minor rearrangement of the domains.

==About this Structure==
2EYN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EYN OCA]. 

==Reference==
Crystal structure of the actin-binding domain of alpha-actinin 1: evaluating two competing actin-binding models., Borrego-Diaz E, Kerff F, Lee SH, Ferron F, Li Y, Dominguez R, J Struct Biol. 2006 Aug;155(2):230-8. Epub 2006 Apr 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16698282 16698282]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Borrego-Diaz, E.]]
[[Category: Dominguez, R.]]
[[Category: Ferron, F.]]
[[Category: Kerff, F.]]
[[Category: Lee, S H.]]
[[Category: Li, Y.]]
[[Category: actin-binding]]
[[Category: actin-bundling]]
[[Category: actin-crosslinking]]
[[Category: calponin homology domain]]
[[Category: ch domain]]
[[Category: structural protein]]

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