View source for 2e5t

[[Image:2e5t.jpg|left|200px]]<br /><applet load="2e5t" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="2e5t" />
'''C-terminal domain of Epsilon subunit of F1F0-ATP synthase from the Thermophilic bacillus PS3 in the presence of ATP condition'''<br />

==Overview==
The epsilon subunit of bacterial and chloroplast F(o)F(1)-ATP synthases modulates their ATP hydrolysis activity. Here, we report the crystal structure of the ATP-bound epsilon subunit from a thermophilic Bacillus PS3 at 1.9-A resolution. The C-terminal two alpha-helices were folded into a hairpin, sitting on the beta sandwich structure, as reported for Escherichia coli. A previously undescribed ATP binding motif, I(L)DXXRA, recognizes ATP together with three arginine and one glutamate residues. The E. coli epsilon subunit binds ATP in a similar manner, as judged on NMR. We also determined solution structures of the C-terminal domain of the PS3 epsilon subunit and relaxation parameters of the whole molecule by NMR. The two helices fold into a hairpin in the presence of ATP but extend in the absence of ATP. The latter structure has more helical regions and is much more flexible than the former. These results suggest that the epsilon C-terminal domain can undergo an arm-like motion in response to an ATP concentration change and thereby contribute to regulation of F(o)F(1)-ATP synthase.

==About this Structure==
2E5T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_sp._ps3 Bacillus sp. ps3]. Active as [http://en.wikipedia.org/wiki/H(+)-transporting_two-sector_ATPase H(+)-transporting two-sector ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.14 3.6.3.14] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E5T OCA]. 

==Reference==
Structures of the thermophilic F1-ATPase epsilon subunit suggesting ATP-regulated arm motion of its C-terminal domain in F1., Yagi H, Kajiwara N, Tanaka H, Tsukihara T, Kato-Yamada Y, Yoshida M, Akutsu H, Proc Natl Acad Sci U S A. 2007 Jul 3;104(27):11233-8. Epub 2007 Jun 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17581881 17581881]
[[Category: Bacillus sp. ps3]]
[[Category: H(+)-transporting two-sector ATPase]]
[[Category: Single protein]]
[[Category: Akutsu, H.]]
[[Category: Yagi, H.]]
[[Category: atp]]
[[Category: atp synthase]]
[[Category: epsilon subunit]]
[[Category: f1-atpase]]
[[Category: f1fo atp synthase]]

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