View source for 2bk0

[[Image:2bk0.gif|left|200px]]<br /><applet load="2bk0" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="2bk0, resolution 2.90&Aring;" />
'''CRYSTAL STRUCTURE OF THE MAJOR CELERY ALLERGEN API G 1'''<br />

==Overview==
Many patients who have been sensitised to pollen, display allergic symptoms after ingestion of certain plant food such as fresh fruit, vegetables and nuts. The cause is the cross-reactivity between structurally very similar major plant allergens. In particular, allergy to celery is very frequently associated with birch and mugwort pollen sensitization, known as to the birch-mugwort-celery syndrome. The crystal structure of the major celery allergen Api g 1, a homologue of the major birch pollen allergen Bet v 1, has been determined to a resolution of 2.9 A. The structure of Api g 1 is very similar to that of Bet v 1 with major differences occurring in the segment comprised of residues 23-45, preceding the well conserved glycine-rich P-loop, as well as in loops beta3-beta4 and beta5-beta6. In particular, Api g 1 lacks E45, which has been shown to be a crucial residue for antibody recognition in the crystal complex of Bet v 1 with the Fab fragment of a murine monoclonal IgG (BV16) antibody. The absence of E45 and the structural differences in the preceding segment suggest that this region of the Api g 1 surface is probably not responsible for the observed cross-reactivity with Bet v 1. A detailed analysis of the molecular surface in combination with sequence alignment revealed three conserved surface patches which may account for cross-reactivity with Bet v 1. Several residues of Bet v 1 which have been shown by mutagenesis studies to be involved in IgE recognition belong to these conserved surface regions. The structure of Api g 1 and the related epitope analysis provides a molecular basis for a better understanding of allergen cross-reactivity and may lead to the development of hypoallergens which would allow a safer immunotherapy.

==About this Structure==
2BK0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Apium_graveolens Apium graveolens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BK0 OCA]. 

==Reference==
Crystal structure of the major celery allergen Api g 1: molecular analysis of cross-reactivity., Schirmer T, Hoffimann-Sommergrube K, Susani M, Breiteneder H, Markovic-Housley Z, J Mol Biol. 2005 Sep 2;351(5):1101-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16051263 16051263]
[[Category: Apium graveolens]]
[[Category: Single protein]]
[[Category: Breiteneder, H.]]
[[Category: Hoffmann-Sommergruber, K.]]
[[Category: Markovic-Housley, Z.]]
[[Category: Schirmer, T.]]
[[Category: allergen]]
[[Category: bet v 1-related protein]]
[[Category: cross reactive epitopes]]
[[Category: major celery allergen api g 1]]
[[Category: pathogenesis-related protein]]
[[Category: plant defense]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:38:42 2008''