View source for 1zui

[[Image:1zui.gif|left|200px]]<br /><applet load="1zui" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="1zui, resolution 2.300&Aring;" />
'''Structural Basis for Shikimate-binding Specificity of Helicobacter pylori Shikimate Kinase'''<br />

==Overview==
Shikimate kinase (EC 2.7.1.71) catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid in the presence of ATP. As the fifth key step in the shikimate pathway for aromatic amino acid biosynthesis in bacteria, fungi, and plants, but not mammals, shikimate kinase represents an attractive target for the development of new antimicrobial agents, herbicides, and antiparasitic agents. Here, we report the 1.8-Angstroms crystal structure of Helicobacter pylori shikimate kinase (HpSK). The crystal structure shows a three-layer alpha/beta fold consisting of a central sheet of five parallel beta-strands flanked by seven alpha-helices. An HpSK-shikimate-PO(4) complex was also determined and refined to 2.3 Angstroms, revealing induced-fit movement from an open to a closed form on substrate binding. Shikimate is located above a short 3(10) helix formed by a strictly conserved motif (GGGXV) after beta(3). Moreover, several highly conserved charged residues including Asp33 (in a conserved DT/SD motif), Arg57, and Arg132 (interacting with shikimate) are identified, guiding the development of novel inhibitors of shikimate kinase.

==About this Structure==
1ZUI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Helicobacter_pylori Helicobacter pylori] with <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=SKM:'>SKM</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Shikimate_kinase Shikimate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.71 2.7.1.71] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZUI OCA]. 

==Reference==
Structural basis for shikimate-binding specificity of Helicobacter pylori shikimate kinase., Cheng WC, Chang YN, Wang WC, J Bacteriol. 2005 Dec;187(23):8156-63. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16291688 16291688]
[[Category: Helicobacter pylori]]
[[Category: Shikimate kinase]]
[[Category: Single protein]]
[[Category: Chang, Y N.]]
[[Category: Cheng, W C.]]
[[Category: Wang, W C.]]
[[Category: PO4]]
[[Category: SKM]]
[[Category: alpha-beta protein]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:19:10 2008''