View source for 1xtq

[[Image:1xtq.gif|left|200px]]<br /><applet load="1xtq" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="1xtq, resolution 2.00&Aring;" />
'''Structure of small GTPase human Rheb in complex with GDP'''<br />

==Overview==
The small GTPase Rheb displays unique biological and biochemical properties different from other small GTPases and functions as an important mediator between the tumor suppressor proteins TSC1 and TSC2 and the mammalian target of rapamycin to stimulate cell growth. We report here the three-dimensional structures of human Rheb in complexes with GDP, GTP, and GppNHp (5'-(beta,gamma-imide)triphosphate), which reveal novel structural features of Rheb and provide a molecular basis for its distinct properties. During GTP/GDP cycling, switch I of Rheb undergoes conformational change while switch II maintains a stable, unusually extended conformation, which is substantially different from the alpha-helical conformation seen in other small GTPases. The unique switch II conformation results in a displacement of Gln64 (equivalent to the catalytic Gln61 of Ras), making it incapable of participating in GTP hydrolysis and thus accounting for the low intrinsic GTPase activity of Rheb. This rearrangement also creates space to accommodate the side chain of Arg15, avoiding its steric hindrance with the catalytic residue and explaining its noninvolvement in GTP hydrolysis. Unlike Ras, the phosphate moiety of GTP in Rheb is shielded by the conserved Tyr35 of switch I, leading to the closure of the GTP-binding site, which appears to prohibit the insertion of a potential arginine finger from its GTPase-activating protein. Taking the genetic, biochemical, biological, and structural data together, we propose that Rheb forms a new group of the Ras/Rap subfamily and uses a novel GTP hydrolysis mechanism that utilizes Asn1643 of the tuberous sclerosis complex 2 GTPase-activating protein domain instead of Gln64 of Rheb as the catalytic residue.

==About this Structure==
1XTQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=GDP:'>GDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XTQ OCA]. 

==Reference==
Structural basis for the unique biological function of small GTPase RHEB., Yu Y, Li S, Xu X, Li Y, Guan K, Arnold E, Ding J, J Biol Chem. 2005 Apr 29;280(17):17093-100. Epub 2005 Feb 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15728574 15728574]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Ding, J.]]
[[Category: Yu, Y.]]
[[Category: GDP]]
[[Category: MG]]
[[Category: beta saddle]]
[[Category: p-loop]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:58:35 2008''