View source for 1h5k

[[Image:1h5k.gif|left|200px]]<br />
<applet load="1h5k" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1h5k, resolution 1.60&Aring;" />
'''X-RAY INDUCED REDUCTION OF HORSERADISH PEROXIDASE C1A COMPOUND III (78-89% DOSE)'''<br />

==Overview==
A molecular description of oxygen and peroxide activation in biological, systems is difficult, because electrons liberated during X-ray data, collection reduce the active centres of redox enzymes catalysing these, reactions. Here we describe an effective strategy to obtain crystal, structures for high-valency redox intermediates and present a, three-dimensional movie of the X-ray-driven catalytic reduction of a bound, dioxygen species in horseradish peroxidase (HRP). We also describe, separate experiments in which high-resolution structures could be obtained, for all five oxidation states of HRP, showing such structures with, preserved redox states for the first time.

==About this Structure==
1H5K is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Armoracia_rusticana Armoracia rusticana]] with ACT, CA, HEM and PEO as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Peroxidase Peroxidase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.7 1.11.1.7]]. Structure known Active Site: CAA. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H5K OCA]]. 

==Reference==
The catalytic pathway of horseradish peroxidase at high resolution., Berglund GI, Carlsson GH, Smith AT, Szoke H, Henriksen A, Hajdu J, Nature. 2002 May 23;417(6887):463-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12024218 12024218]
[[Category: Armoracia rusticana]]
[[Category: Peroxidase]]
[[Category: Single protein]]
[[Category: Berglund, G.I.]]
[[Category: Carlsson, G.H.]]
[[Category: Hajdu, J.]]
[[Category: Henriksen, A.]]
[[Category: Smith, A.T.]]
[[Category: Szoke, H.]]
[[Category: ACT]]
[[Category: CA]]
[[Category: HEM]]
[[Category: PEO]]
[[Category: compound iii]]
[[Category: horseradish]]
[[Category: oxidoreductase]]
[[Category: oxyperoxidase]]
[[Category: peroxidase]]
[[Category: x-ray induced reduction]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:29:35 2007''