View source for 1gn6

[[Image:1gn6.gif|left|200px]]<br />
<applet load="1gn6" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1gn6, resolution 2.9&Aring;" />
'''G152A MUTANT OF MYCOBACTERIUM TUBERCULOSIS IRON-SUPEROXIDE DISMUTASE.'''<br />

==Overview==
We have refined the X-ray structure of a site-directed G152A mutant of the, iron-dependent superoxide dismutase from Mycobacterium tuberculosis at 2.9, angstroms resolution. The mutation which replaces a glycine residue in a, surface loop with alanine was designed to alter the conformation of this, loop region which has previously been shown to play a crucial structural, role in quaternary interactions within the SOD tetramer. Gly-152 was, targeted as it has dihedral angles (phi = 83.1 degrees, psi = -0.3, degrees) close to the left-handed alpha-helical conformation which is, rarely adopted by other amino acids except asparagine. Gly-152 was, replaced by alanine as it has similar size and polarity, yet has a very, low tendency to adopt similar conformations. X-ray data collection on, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?8674528 (full description)]]

==About this Structure==
1GN6 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]] with FE as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Superoxide_dismutase Superoxide dismutase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.15.1.1 1.15.1.1]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GN6 OCA]]. 

==Reference==
X-ray structure analysis of an engineered Fe-superoxide dismutase Gly-Ala mutant with significantly reduced stability to denaturant., Cooper JB, Saward S, Erskine PT, Badasso MO, Wood SP, Zhang Y, Young D, FEBS Lett. 1996 Jun 3;387(2-3):105-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8674528 8674528]
[[Category: Mycobacterium tuberculosis]]
[[Category: Single protein]]
[[Category: Superoxide dismutase]]
[[Category: Badasso, M.O.]]
[[Category: Bunting, K.A.]]
[[Category: Cooper, J.B.]]
[[Category: Erskine, P.T.]]
[[Category: Saward, S.]]
[[Category: Wood, S.P.]]
[[Category: Young, D.B.]]
[[Category: Zhang, Y.]]
[[Category: FE]]
[[Category: oxidoreductase]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:15:34 2007''