View source for 1twf

[[Image:1twf.gif|left|200px]]<br /><applet load="1twf" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="1twf, resolution 2.3&Aring;" />
'''RNA polymerase II complexed with UTP at 2.3 A resolution'''<br />

==Overview==
Binding of a ribonucleoside triphosphate to an RNA polymerase II transcribing complex, with base pairing to the template DNA, was revealed by X-ray crystallography. Binding of a mismatched nucleoside triphosphate was also detected, but in an adjacent site, inverted with respect to the correctly paired nucleotide. The results are consistent with a two-step mechanism of nucleotide selection, with initial binding to an entry (E) site beneath the active center in an inverted orientation, followed by rotation into the nucleotide addition (A) site for pairing with the template DNA. This mechanism is unrelated to that of single subunit RNA polymerases and so defines a new paradigm for the large, multisubunit enzymes. Additional findings from these studies include a third nucleotide binding site that may define the length of backtracked RNA; DNA double helix unwinding in advance of the polymerase active center; and extension of the diffraction limit of RNA polymerase II crystals to 2.3 A.

==About this Structure==
1TWF is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=UTP:'>UTP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TWF OCA]. 

==Reference==
Structural basis of transcription: nucleotide selection by rotation in the RNA polymerase II active center., Westover KD, Bushnell DA, Kornberg RD, Cell. 2004 Nov 12;119(4):481-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15537538 15537538]
[[Category: DNA-directed RNA polymerase]]
[[Category: Protein complex]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Bushnell, D A.]]
[[Category: Kornberg, R D.]]
[[Category: Westover, K D.]]
[[Category: MN]]
[[Category: UTP]]
[[Category: ZN]]
[[Category: molecular machine]]
[[Category: mrna]]
[[Category: multiprotein complex]]
[[Category: transcription]]
[[Category: zinc motifs]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:18:04 2008''