View source for 1t1d

[[Image:1t1d.gif|left|200px]]<br /><applet load="1t1d" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="1t1d, resolution 1.51&Aring;" />
'''CRYSTAL STRUCTURE OF THE TETRAMERIZATION DOMAIN OF THE SHAKER POTASSIUM CHANNEL'''<br />

==Overview==
The N-terminal, cytoplasmic tetramerization domain (T1) of voltage-gated K+ channels encodes molecular determinants for subfamily-specific assembly of alpha-subunits into functional tetrameric channels. Crystal structures of T1 tetramers from Shaw and Shaker subfamilies reveal a common four-layered scaffolding. Within layer 4, on the hypothetical membrane-facing side of the tetramer, the Shaw T1 tetramer contains four zinc ions; each is coordinated by a histidine and two cysteines from one monomer and by one cysteine from an adjacent monomer. The amino acids involved in coordinating the Zn2+ ion occur in a HX5CX20CC sequence motif that is highly conserved among all Shab, Shaw and Shal subfamily members, but is not found in Shaker subfamily members. We demonstrate by coimmunoprecipitation that a few characteristic residues in the subunit interface are crucial for subfamily-specific tetramerization of the T1 domains.

==About this Structure==
1T1D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T1D OCA]. 

==Reference==
Zn2+-binding and molecular determinants of tetramerization in voltage-gated K+ channels., Bixby KA, Nanao MH, Shen NV, Kreusch A, Bellamy H, Pfaffinger PJ, Choe S, Nat Struct Biol. 1999 Jan;6(1):38-43. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9886290 9886290]
[[Category: Aplysia californica]]
[[Category: Single protein]]
[[Category: Choe, S.]]
[[Category: Kreusch, A.]]
[[Category: Pfaffinger, P J.]]
[[Category: Stevens, C F.]]
[[Category: aplysia kv1 1]]
[[Category: potassium channels]]
[[Category: proton transport]]
[[Category: tetramerization domain]]
[[Category: x-ray structure]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:08:53 2008''