View source for 1rgi

[[Image:1rgi.gif|left|200px]]<br /><applet load="1rgi" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="1rgi, resolution 3.00&Aring;" />
'''Crystal structure of gelsolin domains G1-G3 bound to actin'''<br />

==Overview==
The actin filament-severing functionality of gelsolin resides in its N-terminal three domains (G1-G3). We have determined the structure of this fragment in complex with an actin monomer. The structure reveals the dramatic domain rearrangements that activate G1-G3, which include the replacement of interdomain interactions observed in the inactive, calcium-free protein by new contacts to actin, and by a novel G2-G3 interface. Together, these conformational changes are critical for actin filament severing, and we suggest that their absence leads to the disease Finnish-type familial amyloidosis. Furthermore, we propose that association with actin drives the calcium-independent activation of isolated G1-G3 during apoptosis, and that a similar mechanism operates to activate native gelsolin at micromolar levels of calcium. This is the first structure of a filament-binding protein bound to actin and it sets stringent, high-resolution limitations on the arrangement of actin protomers within the filament.

==About this Structure==
1RGI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Equus_caballus Equus caballus] and [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=ATP:'>ATP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RGI OCA]. 

==Reference==
Structure of the N-terminal half of gelsolin bound to actin: roles in severing, apoptosis and FAF., Burtnick LD, Urosev D, Irobi E, Narayan K, Robinson RC, EMBO J. 2004 Jul 21;23(14):2713-22. Epub 2004 Jun 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15215896 15215896]
[[Category: Equus caballus]]
[[Category: Oryctolagus cuniculus]]
[[Category: Protein complex]]
[[Category: Burtnick, L D.]]
[[Category: Irobi, E.]]
[[Category: Narayan, K.]]
[[Category: Robinson, R C.]]
[[Category: Urosev, D.]]
[[Category: ATP]]
[[Category: CA]]
[[Category: domain movement]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:50:39 2008''