View source for 1ppf

[[Image:1ppf.gif|left|200px]]<br /><applet load="1ppf" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="1ppf, resolution 1.8&Aring;" />
'''X-RAY CRYSTAL STRUCTURE OF THE COMPLEX OF HUMAN LEUKOCYTE ELASTASE (PMN ELASTASE) AND THE THIRD DOMAIN OF THE TURKEY OVOMUCOID INHIBITOR'''<br />

==Overview==
Orthorhombic crystals diffracting beyond 1.7 A resolution, have been grown from the stoichiometric complex formed between human leukocyte elastase (HLE) and the third domain of turkey ovomucoid inhibitor (OMTKY3). The crystal and molecular structure has been determined with the multiple isomorphous replacement technique. The complex has been modeled using the known structure of OMTKY3 and partial sequence information for HLE, and has been refined. The current crystallographic R-value is 0.21 for reflections from 25 to 1.8 A resolution. HLE shows the characteristic polypeptide fold of trypsin-like serine proteinases and consists of 218 amino acid residues. However, several loop segments, mainly arranged around the substrate binding site, have unique conformations. The largest deviations from the other vertebrate proteinases of known spatial structure are around Cys168. The specificity pocket is constricted by Val190, Val216 and Asp226 to preferentially accommodate medium sized hydrophobic amino acids at P1. Seven residues of the OMTKY3-binding segment are in specific contact with HLE. This interaction and geometry around the reactive site are similar as observed in other complexes. It is the first serine proteinase glycoprotein analysed, having two sugar chains attached to Asn159 and to residue 109.

==Disease==
Known diseases associated with this structure: Hematopoiesis, cyclic OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=130130 130130]], Neutropenia, congenital OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=130130 130130]]

==About this Structure==
1PPF is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Active as [http://en.wikipedia.org/wiki/Leukocyte_elastase Leukocyte elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.37 3.4.21.37] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PPF OCA]. 

==Reference==
X-ray crystal structure of the complex of human leukocyte elastase (PMN elastase) and the third domain of the turkey ovomucoid inhibitor., Bode W, Wei AZ, Huber R, Meyer E, Travis J, Neumann S, EMBO J. 1986 Oct;5(10):2453-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=3640709 3640709]
[[Category: Leukocyte elastase]]
[[Category: Protein complex]]
[[Category: Bode, W.]]
[[Category: Wei, A-Z.]]
[[Category: hydrolase(serine proteinase)]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:31:07 2008''