View source for 1as7

[[Image:1as7.gif|left|200px]]<br />
<applet load="1as7" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1as7, resolution 2.0&Aring;" />
'''STRUCTURE OF ALCALIGENES FAECALIS NITRITE REDUCTASE AT CRYO TEMPERATURE'''<br />

==Overview==
The structures of oxidized, reduced, nitrite-soaked oxidized and, nitrite-soaked reduced nitrite reductase from Alcaligenes faecalis have, been determined at 1.8-2.0 A resolution using data collected at -160, degrees C. The active site at cryogenic temperature, as at room, temperature, contains a tetrahedral type II copper site liganded by three, histidines and a water molecule. The solvent site is empty when crystals, are reduced with ascorbate. A fully occupied oxygen-coordinate nitrite, occupies the solvent site in crystals soaked in nitrite. Ascorbate-reduced, crystals soaked in a glycerol-methanol solution and nitrite at -40 degrees, C remain colorless at -160 degrees C but turn amber-brown when warmed, suggesting that NO is released. Nitrite is found at one-half occupancy., Five new ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9353305 (full description)]]

==About this Structure==
1AS7 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]] with CU as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Transferred_entry:_1.7.2.1 Transferred entry: 1.7.2.1]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.99.3 1.7.99.3]]. Structure known Active Sites: CU1, CU2, CU3, CU4, CU5 and CU6. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AS7 OCA]]. 

==Reference==
Structure of nitrite bound to copper-containing nitrite reductase from Alcaligenes faecalis. Mechanistic implications., Murphy ME, Turley S, Adman ET, J Biol Chem. 1997 Nov 7;272(45):28455-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9353305 9353305]
[[Category: Alcaligenes faecalis]]
[[Category: Single protein]]
[[Category: Transferred entry: 1.7.2.1]]
[[Category: Adman, E.T.]]
[[Category: Murphy, M.E.P.]]
[[Category: Turley, S.]]
[[Category: CU]]
[[Category: copper]]
[[Category: denitrification]]
[[Category: nitrite]]
[[Category: oxidoreductase]]

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