View source for 1lui

[[Image:1lui.jpg|left|200px]]<br /><applet load="1lui" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="1lui" />
'''NMR Structures of Itk SH2 domain, Pro287cis isoform, ensemble of 20 low energy structures'''<br />

==Overview==
Interleukin-2 tyrosine kinase (Itk) is a T cell-specific kinase required for a proper immune response following T cell receptor engagement. In addition to the kinase domain, Itk is composed of several noncatalytic regulatory domains, including a Src homology 2 (SH2) domain that contains a conformationally heterogeneous Pro residue. Cis-trans isomerization of a single prolyl imide bond within the SH2 domain mediates conformer-specific ligand recognition that may have functional implications in T cell signaling. To better understand the mechanism by which a proline switch regulates ligand binding, we have used NMR spectroscopy to determine two structures of Itk SH2 corresponding to the cis and trans imide bond-containing conformers. The structures indicate that the heterogeneous Pro residue acts as a hinge that modulates ligand recognition by controlling the relative orientation of protein-binding surfaces.

==About this Structure==
1LUI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=ACE:'>ACE</scene> and <scene name='pdbligand=NH2:'>NH2</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Transferase Transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.10.1 and 2.7.10.2 2.7.10.1 and 2.7.10.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LUI OCA]. 

==Reference==
Structural characterization of a proline-driven conformational switch within the Itk SH2 domain., Mallis RJ, Brazin KN, Fulton DB, Andreotti AH, Nat Struct Biol. 2002 Dec;9(12):900-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12402030 12402030]
[[Category: Mus musculus]]
[[Category: Single protein]]
[[Category: Transferase]]
[[Category: Andreotti, A M.]]
[[Category: Brazin, K N.]]
[[Category: Fulton, B F.]]
[[Category: Mallis, R J.]]
[[Category: ACE]]
[[Category: NH2]]
[[Category: cis/trans isomerization]]
[[Category: itk]]
[[Category: proline]]
[[Category: sh2 domain]]
[[Category: t-cell]]
[[Category: tsk]]

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