View source for 1llw

[[Image:1llw.gif|left|200px]]<br /><applet load="1llw" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="1llw, resolution 2.70&Aring;" />
'''Structural studies on the synchronization of catalytic centers in glutamate synthase: complex with 2-oxoglutarate'''<br />

==Overview==
The complex iron-sulfur flavoprotein glutamate synthase (GltS) plays a prominent role in ammonia assimilation in bacteria, yeasts, and plants. GltS catalyzes the formation of two molecules of l-glutamate from 2-oxoglutarate and l-glutamine via intramolecular channeling of ammonia. GltS has the impressive ability of synchronizing its distinct catalytic centers to avoid wasteful consumption of l-glutamine. We have determined the crystal structure of the ferredoxin-dependent GltS in several ligation and redox states. The structures reveal the crucial elements in the synchronization between the glutaminase site and the 2-iminoglutarate reduction site. The structural data combined with the catalytic properties of GltS indicate that binding of ferredoxin and 2-oxoglutarate to the FMN-binding domain of GltS induce a conformational change in the loop connecting the two catalytic centers. The rearrangement induces a shift in the catalytic elements of the amidotransferase domain, such that it becomes activated. This machinery, over a distance of more than 30 A, controls the ability of the enzyme to bind and hydrolyze the ammonia-donating substrate l-glutamine.

==About this Structure==
1LLW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Synechocystis_sp. Synechocystis sp.] with <scene name='pdbligand=FMN:'>FMN</scene>, <scene name='pdbligand=F3S:'>F3S</scene> and <scene name='pdbligand=AKG:'>AKG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Glutamate_synthase_(ferredoxin) Glutamate synthase (ferredoxin)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.7.1 1.4.7.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LLW OCA]. 

==Reference==
Structural studies on the synchronization of catalytic centers in glutamate synthase., van den Heuvel RH, Ferrari D, Bossi RT, Ravasio S, Curti B, Vanoni MA, Florencio FJ, Mattevi A, J Biol Chem. 2002 Jul 5;277(27):24579-83. Epub 2002 Apr 19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11967268 11967268]
[[Category: Glutamate synthase (ferredoxin)]]
[[Category: Single protein]]
[[Category: Synechocystis sp.]]
[[Category: Bossi, R T.]]
[[Category: Curti, B.]]
[[Category: Ferrari, D.]]
[[Category: Florencio, F J.]]
[[Category: Heuvel, R H.van den.]]
[[Category: Mattevi, A.]]
[[Category: Ravasio, S.]]
[[Category: Vanoni, M A.]]
[[Category: AKG]]
[[Category: F3S]]
[[Category: FMN]]
[[Category: chanelling]]
[[Category: glutamate synthase]]
[[Category: ntn amidotransferase]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:46:10 2008''