View source for 2ux6

[[Image:2ux6.gif|left|200px]]<br />
<applet load="2ux6" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="2ux6, resolution 1.30&Aring;" />
'''PSEUDOAZURIN WITH ENGINEERED AMICYANIN LIGAND LOOP, OXIDIZED FORM, PH 7.5'''<br />

==Overview==
Atomic resolution structures of the pseudoazurin (PAZ) variant into which, the shorter ligand-containing loop of amicyanin (AMI) is introduced have, been determined. The mutated loop adopts a different conformation in, PAZAMI than in AMI. The copper site structure is affected, with the major, influence being an increased axial interaction resulting in the shortest, Cu(II)-S(Met) bond observed for the cupredoxin family of electron-transfer, proteins. This is accompanied by a lengthening of the important Cu-S(Cys), bond and enhanced tetragonal distortion, consistent with the influence of, the PAZAMI loop contraction on the UV/vis spectrum. The change in active, site geometry is the major cause of the 50 mV decrease in reduction, potential. The copper site structure changes very little upon ... [[http://ispc.weizmann.ac.il/pmbin/getpm?17685636 (full description)]]

==About this Structure==
2UX6 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Achromobacter_cycloclastes Achromobacter cycloclastes]] with CU, CL and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2UX6 OCA]]. 

==Reference==
Influence of Loop Shortening on the Metal Binding Site of Cupredoxin Pseudoazurin(,)., Velarde M, Huber R, Yanagisawa S, Dennison C, Messerschmidt A, Biochemistry. 2007 Aug 9;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17685636 17685636]
[[Category: Achromobacter cycloclastes]]
[[Category: Single protein]]
[[Category: Dennison, C.]]
[[Category: Huber, R.]]
[[Category: Messerschmidt, A.]]
[[Category: Velarde, M.]]
[[Category: Yanagisawa, S.]]
[[Category: CL]]
[[Category: CU]]
[[Category: GOL]]
[[Category: copper]]
[[Category: cupredoxin]]
[[Category: electron transfer]]
[[Category: electron transport]]
[[Category: loop shortening]]
[[Category: metal-binding]]
[[Category: periplasmic]]
[[Category: protein scaffold]]
[[Category: redox potential]]
[[Category: spectroscopic properties]]
[[Category: transport]]
[[Category: type-1 copper]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:37:30 2007''