View source for 1ckn

[[Image:1ckn.gif|left|200px]]<br />
<applet load="1ckn" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1ckn, resolution 2.5&Aring;" />
'''STRUCTURE OF GUANYLYLATED MRNA CAPPING ENZYME COMPLEXED WITH GTP'''<br />

==Overview==
We have solved the crystal structure of an mRNA capping enzyme at 2.5 A, resolution. The enzyme comprises two domains with a deep, but narrow, cleft between them. The two molecules in the crystallographic asymmetric, unit adopt very different conformations; both contain a bound GTP, but one, protein molecule is in an open conformation while the other is in a closed, conformation. Only in the closed conformation is the enzyme able to bind, manganese ions and undergo catalysis within the crystals to yield the, covalent guanylated enzyme intermediate. These structures provide direct, evidence for a mechanism that involves a significant conformational change, in the enzyme during catalysis.

==About this Structure==
1CKN is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Paramecium_bursaria_chlorella_virus_1 Paramecium bursaria chlorella virus 1]] with MN, SO4 and GTP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/mRNA_guanylyltransferase mRNA guanylyltransferase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.50 2.7.7.50]]. Structure known Active Sites: GMA and GMB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CKN OCA]]. 

==Reference==
X-ray crystallography reveals a large conformational change during guanyl transfer by mRNA capping enzymes., Hakansson K, Doherty AJ, Shuman S, Wigley DB, Cell. 1997 May 16;89(4):545-53. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9160746 9160746]
[[Category: Paramecium bursaria chlorella virus 1]]
[[Category: Single protein]]
[[Category: mRNA guanylyltransferase]]
[[Category: Doherty, A.J.]]
[[Category: Hakansson, K.]]
[[Category: Wigley, D.B.]]
[[Category: GTP]]
[[Category: MN]]
[[Category: SO4]]
[[Category: capping enzyme]]
[[Category: mrna]]
[[Category: nucleotidyltransferase]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:35:19 2007''