View source for 1knx

[[Image:1knx.gif|left|200px]]<br /><applet load="1knx" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="1knx, resolution 2.50&Aring;" />
'''HPr kinase/phosphatase from Mycoplasma pneumoniae'''<br />

==Overview==
HPr kinase/phosphatase (HPrK/P) modifies serine 46 of histidine-containing protein (HPr), the phosphorylation state of which is the control point of carbon catabolite repression in low G+C Gram-positive bacteria. To understand the structural mechanism by which HPrK/P carries out its dual, competing activities we determined the structure of full length HPrK/P from Mycoplasma pneumoniae (PD8 ID, 1KNX) to 2.5A resolution. The enzyme forms a homo-hexamer with each subunit containing two domains connected by a short loop. The C-terminal domain contains the well-described P-loop (Walker A box) ATP binding motif and takes a fold similar to phosphoenolpyruvate carboxykinase (PEPCK) from Escherichia coli as recently described in other HPrK/P structures. As expected, the C-terminal domain is very similar to the C-terminal fragment of Lactobacillus casei HPrK/P and the C-terminal domain of Staphylococcus xylosus HPrK/P; the N-terminal domain is very similar to the N-terminal domain of S.xylosus HPrK/P. Unexpectedly, the N-terminal domain resembles UDP-N-acetylmuramoyl-L-alanyl-D-glutamate:meso-diaminopimelate ligase (MurE), yet the function of this domain is unclear. We discuss these observations as well as the structural significance of mutations in the P-loop and HPrK/P family sequence motif.

==About this Structure==
1KNX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycoplasma_pneumoniae Mycoplasma pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KNX OCA]. 

==Reference==
Crystal structure of HPr kinase/phosphatase from Mycoplasma pneumoniae., Allen GS, Steinhauer K, Hillen W, Stulke J, Brennan RG, J Mol Biol. 2003 Feb 28;326(4):1203-17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12589763 12589763]
[[Category: Mycoplasma pneumoniae]]
[[Category: Single protein]]
[[Category: Allen, G S.]]
[[Category: catabolite repression]]
[[Category: hpr kinase]]
[[Category: hpr kinase/phosphatase]]
[[Category: hprk/p]]
[[Category: kinase]]
[[Category: p-loop]]
[[Category: phosphatase]]
[[Category: walker a box]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:36:06 2008''