View source for 1ome

[[Image:1ome.gif|left|200px]]<br />
<applet load="1ome" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1ome, resolution 2.3&Aring;" />
'''CRYSTAL STRUCTURE OF THE OMEGA LOOP DELETION MUTANT (RESIDUES 163-178 DELETED) OF BETA-LACTAMASE FROM STAPHYLOCOCCUS AUREUS PC1'''<br />

==Overview==
The structure of class A beta-lactamases contains an omega-loop associated, with the active site, which carries a key catalytic residue, Glu166. A, 16-residue omega-loop deletion mutant of beta-lactamase from, Staphylococcus aureus PC1, encompassing residues 163-178, was produced in, order to examine the functional and structural role of the loop. The, crystal structure was determined and refined at 2.3 A, and the kinetics of, the mutant enzyme was characterized with a variety of beta-lactam, antibiotics. In general, the wild-type beta-lactamase hydrolyzes, penicillin compounds better than cephalosporins. In contrast, the deletion, of the omega-loop led to a variant enzyme that acts only on, cephalosporins, including third generation compounds. Kinetic measurements, and electrospray mass ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9521648 (full description)]]

==About this Structure==
1OME is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]] with CL as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6]]. Structure known Active Site: CAT. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OME OCA]]. 

==Reference==
Role of the omega-loop in the activity, substrate specificity, and structure of class A beta-lactamase., Banerjee S, Pieper U, Kapadia G, Pannell LK, Herzberg O, Biochemistry. 1998 Mar 10;37(10):3286-96. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9521648 9521648]
[[Category: Beta-lactamase]]
[[Category: Single protein]]
[[Category: Staphylococcus aureus]]
[[Category: Banerjee, S.]]
[[Category: Herzberg, O.]]
[[Category: Pieper, U.]]
[[Category: CL]]
[[Category: beta-lactam antibiotic resistance]]
[[Category: beta-lactamase]]
[[Category: hydrolase]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:29:22 2007''