View source for 1jpe

[[Image:1jpe.jpg|left|200px]]<br /><applet load="1jpe" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="1jpe, resolution 1.90&Aring;" />
'''Crystal structure of DsbD-alpha; the N-terminal domain of DsbD'''<br />

==Overview==
The Escherichia coli disulfide bond isomerase DsbC rearranges incorrect disulfide bonds during oxidative protein folding. It is specifically activated by the periplasmic N-terminal domain (DsbDalpha) of the transmembrane electron transporter DsbD. An intermediate of the electron transport reaction was trapped, yielding a covalent DsbC-DsbDalpha complex. The 2.3 A crystal structure of the complex shows for the first time the specific interactions between two thiol oxidoreductases. DsbDalpha is a novel thiol oxidoreductase with the active site cysteines embedded in an immunoglobulin fold. It binds into the central cleft of the V-shaped DsbC dimer, which assumes a closed conformation on complex formation. Comparison of the complex with oxidized DsbDalpha reveals major conformational changes in a cap structure that regulates the accessibility of the DsbDalpha active site. Our results explain how DsbC is selectively activated by DsbD using electrons derived from the cytoplasm.

==About this Structure==
1JPE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JPE OCA]. 

==Reference==
The disulfide bond isomerase DsbC is activated by an immunoglobulin-fold thiol oxidoreductase: crystal structure of the DsbC-DsbDalpha complex., Haebel PW, Goldstone D, Katzen F, Beckwith J, Metcalf P, EMBO J. 2002 Sep 16;21(18):4774-84. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12234918 12234918]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Goldstone, D.]]
[[Category: Haebel, P W.]]
[[Category: Metcalf, P.]]
[[Category: disulfide bond formation]]
[[Category: electron transport]]
[[Category: inner membrane]]
[[Category: redox-active center]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:25:13 2008''