View source for 1fon

[[Image:1fon.gif|left|200px]]<br /><applet load="1fon" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="1fon, resolution 1.7&Aring;" />
'''CRYSTAL STRUCTURE OF BOVINE PROCARBOXYPEPTIDASE A-S6 SUBUNIT III, A HIGHLY STRUCTURED TRUNCATED ZYMOGEN E'''<br />

==Overview==
Subunit III, a defective serine endopeptidase lacking the typical N-terminal hydrophobic dipeptide is secreted by the pancreas of ruminant species as part of the bovine ternary complex procarboxypeptidase A-S6. Two monoclinic crystal forms were obtained and subsequently used to solve its X-ray structure. The highest resolution model of subunit III was refined at 1.7 A resolution to a crystallographic R-factor of 18.4%, with r.m.s. bond deviations from ideality of 0.012 A. About 80% of the model presents the characteristic architecture of trypsin-like proteases. The remaining zones, however, have well-defined, unique conformations. The regions from residues 70 to 80 and from 140 to 155 present maximum distances of 16 and 18 A relative to serine proteases and zymogens. Comparisons with the structures of porcine elastase 1 and chymotrypsinogen A indicate that the specific binding pocket of subunit III adopts a zymogen-like conformation and thus provide a basis for its inactivity. In general, the structural analysis of subunit III strongly suggests that it corresponds to a truncated version of a new class of highly structured elastase-like zymogen molecules. Based on the structures of subunit III and elastase 1, it is concluded that large concerted movements are necessary for the activation of zymogen E.

==About this Structure==
1FON is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FON OCA]. 

==Reference==
Crystal structure of bovine procarboxypeptidase A-S6 subunit III, a highly structured truncated zymogen E., Pignol D, Gaboriaud C, Michon T, Kerfelec B, Chapus C, Fontecilla-Camps JC, EMBO J. 1994 Apr 15;13(8):1763-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8168476 8168476]
[[Category: Bos taurus]]
[[Category: Single protein]]
[[Category: Chapus, C.]]
[[Category: Fontecilla-Camps, J C.]]
[[Category: Gaboriaud, T.]]
[[Category: Kerfelec, B.]]
[[Category: Michon, B.]]
[[Category: Pignol, D C.]]
[[Category: serine protease]]
[[Category: truncated zymogen e]]

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