View source for 1eoe

[[Image:1eoe.jpg|left|200px]]<br /><applet load="1eoe" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="1eoe, resolution 1.704&Aring;" />
'''CRYSTAL STRUCTURE OF THE V135R MUTANT OF A SHAKER T1 DOMAIN'''<br />

==Overview==
The T1 domain, a highly conserved cytoplasmic portion at the N-terminus of the voltage-dependent K+ channel (Kv) alpha-subunit, is responsible for driving and regulating the tetramerization of the alpha-subunits. Here we report the identification of a set of mutations in the T1 domain that alter the gating properties of the Kv channel. Two mutants produce a leftward shift in the activation curve and slow the channel closing rate while a third mutation produces a rightward shift in the activation curve and speeds the channel closing rate. We have determined the crystal structures of T1 domains containing these mutations. Both of the leftward shifting mutants produce similar conformational changes in the putative membrane facing surface of the T1 domain. These results suggest that the structure of the T1 domain in this region is tightly coupled to the channel's gating states.

==About this Structure==
1EOE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EOE OCA]. 

==Reference==
Voltage dependent activation of potassium channels is coupled to T1 domain structure., Cushman SJ, Nanao MH, Jahng AW, DeRubeis D, Choe S, Pfaffinger PJ, Nat Struct Biol. 2000 May;7(5):403-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10802739 10802739]
[[Category: Aplysia californica]]
[[Category: Single protein]]
[[Category: Choe, S.]]
[[Category: Cushman, S J.]]
[[Category: DeRubeis, D.]]
[[Category: Jahng, A W.]]
[[Category: Nanao, M H.]]
[[Category: Pfaffinger, P J.]]
[[Category: aplysia kv1 1]]
[[Category: potassium channels]]
[[Category: proton transport]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:29:53 2008''