View source for 3dap

[[Image:3dap.gif|left|200px]]<br />
<applet load="3dap" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="3dap, resolution 2.2&Aring;" />
'''C. GLUTAMICUM DAP DEHYDROGENASE IN COMPLEX WITH NADP+ AND THE INHIBITOR 5S-ISOXAZOLINE'''<br />

==Overview==
The three-dimensional structures of Corynebacterium glutamicum, diaminopimelate dehydrogenase as a binary complex with the substrate, meso-diaminopimelate (meso-DAP) and a ternary complex with NADP+ and an, isoxazoline inhibitor [Abbot, S.D., Lane-Bell, P., Kanwar, P.S.S., and, Vederas, J. C. (1994) J. Am. Chem. Soc. 116, 6513-6520] have been solved, and refined against X-ray diffraction data to 2.2 A. Diaminopimelate, dehydrogenase is a homodimer of approximately 35,000 molecular weight, subunits and is the only dehydrogenase present in the bacterial, diaminopimelate/lysine biosynthetic pathway. Inhibitors of the enzymes of, L-lysine biosynthesis have been proposed as potential antibiotics or, herbicides, since mammals lack this metabolic pathway. Diaminopimelate, dehydrogenase catalyzes ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9521647 (full description)]]

==About this Structure==
3DAP is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Corynebacterium_glutamicum Corynebacterium glutamicum]] with NDP and DA3 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Diaminopimelate_dehydrogenase Diaminopimelate dehydrogenase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.1.16 1.4.1.16]]. Structure known Active Sites: INH, N1 and N2. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=3DAP OCA]]. 

==Reference==
Substrate and inhibitor binding sites in Corynebacterium glutamicum diaminopimelate dehydrogenase., Scapin G, Cirilli M, Reddy SG, Gao Y, Vederas JC, Blanchard JS, Biochemistry. 1998 Mar 10;37(10):3278-85. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9521647 9521647]
[[Category: Corynebacterium glutamicum]]
[[Category: Diaminopimelate dehydrogenase]]
[[Category: Single protein]]
[[Category: Blanchard, J.S.]]
[[Category: Cirilli, M.]]
[[Category: Gao, Y.]]
[[Category: Reddy, S.G.]]
[[Category: Scapin, G.]]
[[Category: Vederas, J.C.]]
[[Category: DA3]]
[[Category: NDP]]
[[Category: asymmetric dimer]]
[[Category: d-amino acid dehydrogenase]]
[[Category: dehydrogenase]]
[[Category: inhibitor]]
[[Category: lysine biosynthesis]]
[[Category: nadp]]
[[Category: oxidoreductase]]

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