View source for 1clq

[[Image:1clq.gif|left|200px]]<br /><applet load="1clq" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="1clq, resolution 2.70&Aring;" />
'''CRYSTAL STRUCTURE OF A REPLICATION FORK DNA POLYMERASE EDITING COMPLEX AT 2.7 A RESOLUTION'''<br />

==Overview==
We have solved the crystal structures of the bacteriophage RB69 sliding clamp, its complex with a peptide essential for DNA polymerase interactions, and the DNA polymerase complexed with primer-template DNA. The editing complex structure shows a partially melted duplex DNA exiting from the exonuclease domain at an unexpected angle and significant changes in the protein structure. The clamp complex shows the C-terminal 11 residues of polymerase bound in a hydrophobic pocket, and it allows docking of the editing and clamp structures together. The peptide binds to the sliding clamp at a position identical to that of a replication inhibitor peptide bound to PCNA, suggesting that the replication inhibitor protein p21CIP1 functions by competing with eukaryotic polymerases for the same binding pocket on the clamp.

==About this Structure==
1CLQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_rb18 Enterobacteria phage rb18] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=GDP:'>GDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1CLQ with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb3_1.html DNA Polymerase]]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLQ OCA]. 

==Reference==
Building a replisome from interacting pieces: sliding clamp complexed to a peptide from DNA polymerase and a polymerase editing complex., Shamoo Y, Steitz TA, Cell. 1999 Oct 15;99(2):155-66. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10535734 10535734]
[[Category: DNA Polymerase]]
[[Category: DNA-directed DNA polymerase]]
[[Category: Enterobacteria phage rb18]]
[[Category: Single protein]]
[[Category: Shamoo, Y.]]
[[Category: Steitz, T A.]]
[[Category: CA]]
[[Category: GDP]]
[[Category: dna polymerase]]
[[Category: editing]]
[[Category: gp43]]
[[Category: proofreading]]
[[Category: replication]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:07:19 2008''