View source for 1amh

[[Image:1amh.jpg|left|200px]]<br /><applet load="1amh" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="1amh, resolution 2.5&Aring;" />
'''UNCOMPLEXED RAT TRYPSIN MUTANT WITH ASP 189 REPLACED WITH SER (D189S)'''<br />

==Overview==
Structure-based mutational analysis of serine protease specificity has produced a large database of information useful in addressing biological function and in establishing a basis for targeted design efforts. Critical issues examined include the function of water molecules in providing strength and specificity of binding, the extent to which binding subsites are interdependent, and the roles of polypeptide chain flexibility and distal structural elements in contributing to specificity profiles. The studies also provide a foundation for exploring why specificity modification can be either straightforward or complex, depending on the particular system.

==About this Structure==
1AMH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_rattus Rattus rattus] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] Known structural/functional Sites: <scene name='pdbsite=CAA:Catalytic+Site'>CAA</scene> and <scene name='pdbsite=CAB:Catalytic+Site'>CAB</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AMH OCA]. 

==Reference==
Structural basis of substrate specificity in the serine proteases., Perona JJ, Craik CS, Protein Sci. 1995 Mar;4(3):337-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7795518 7795518]
[[Category: Rattus rattus]]
[[Category: Single protein]]
[[Category: Trypsin]]
[[Category: Bocskei, Z S.]]
[[Category: Graf, L.]]
[[Category: Naray-Szabo, G.]]
[[Category: Szabo, E.]]
[[Category: CA]]
[[Category: activation domain]]
[[Category: serine protease]]
[[Category: substrate specificity hydrolase]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:46:07 2008''