View source for 1ad3

[[Image:1ad3.gif|left|200px]]<br /><applet load="1ad3" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="1ad3, resolution 2.6&Aring;" />
'''CLASS 3 ALDEHYDE DEHYDROGENASE COMPLEX WITH NICOTINAMIDE-ADENINE-DINUCLEOTIDE'''<br />

==Overview==
The first structure of an aldehyde dehydrogenase (ALDH) is described at 2.6 A resolution. Each subunit of the dimeric enzyme contains an NAD-binding domain, a catalytic domain and a bridging domain. At the interface of these domains is a 15 A long funnel-shaped passage with a 6 x 12 A opening leading to a putative catalytic pocket. A new mode of NAD binding, which differs substantially from the classic beta-alpha-beta binding mode associated with the 'Rossmann fold', is observed which we term the beta-alpha,beta mode. Sequence comparisons of the class 3 ALDH with other ALDHs indicate a similar polypeptide fold, novel NAD-binding mode and catalytic site for this family. A mechanism for enzymatic specificity and activity is postulated.

==About this Structure==
1AD3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=NAD:'>NAD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Aldehyde_dehydrogenase_(NAD(P)(+)) Aldehyde dehydrogenase (NAD(P)(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.5 1.2.1.5] Known structural/functional Sites: <scene name='pdbsite=CTA:Catalytic+CYS'>CTA</scene> and <scene name='pdbsite=CTB:Catalytic+CYS'>CTB</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AD3 OCA]. 

==Reference==
The first structure of an aldehyde dehydrogenase reveals novel interactions between NAD and the Rossmann fold., Liu ZJ, Sun YJ, Rose J, Chung YJ, Hsiao CD, Chang WR, Kuo I, Perozich J, Lindahl R, Hempel J, Wang BC, Nat Struct Biol. 1997 Apr;4(4):317-26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9095201 9095201]
[[Category: Aldehyde dehydrogenase (NAD(P)(+))]]
[[Category: Rattus norvegicus]]
[[Category: Single protein]]
[[Category: Liu, Z J.]]
[[Category: Rose, J.]]
[[Category: Wang, B C.]]
[[Category: NAD]]
[[Category: aromatic aldehyde]]
[[Category: nadp]]
[[Category: oxidoreductase]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:43:15 2008''