View source for 1v07

[[Image:1v07.gif|left|200px]]<br />
<applet load="1v07" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1v07, resolution 1.70&Aring;" />
'''CRYSTAL STRUCTURE OF THRE11VAL MUTANT OF THE NERVE TISSUE MINI-HEMOGLOBIN FROM THE NEMERTEAN WORM CEREBRATULUS LACTEUS'''<br />

==Overview==
The mini-hemoglobin from Cerebratulus lacteus (CerHb) belongs to a class, of globins containing the polar Tyr-B10/Gln-E7 amino acid pair that, normally causes low rates of O2 dissociation and ultra-high O2 affinity, which suggest O2 sensing or NO scavenging functions. CerHb, however, has, high rates of O2 dissociation (kO2 = 200-600 s(-1)) and moderate O2, affinity (KO2) approximately 1 microm(-1)) as a result of a third polar, amino acid in its active site, Thr-E11. When Thr-E11 is replaced by Val, kO2 decreases 1000-fold and KO2 increases 130-fold at pH 7.0, 20 degrees, C. The mutation also shifts the stretching frequencies of both heme-bound, and photodissociated CO, indicating marked changes of the electrostatic, field at the active site. The crystal structure of Thr-E11 --&gt; Val ... [[http://ispc.weizmann.ac.il/pmbin/getpm?15161908 (full description)]]

==About this Structure==
1V07 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Cerebratulus_lacteus Cerebratulus lacteus]] with SO4, HEM and OXY as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1V07 OCA]]. 

==Reference==
Thr-E11 regulates O2 affinity in Cerebratulus lacteus mini-hemoglobin., Pesce A, Nardini M, Ascenzi P, Geuens E, Dewilde S, Moens L, Bolognesi M, Riggs AF, Hale A, Deng P, Nienhaus GU, Olson JS, Nienhaus K, J Biol Chem. 2004 Aug 6;279(32):33662-72. Epub 2004 May 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15161908 15161908]
[[Category: Cerebratulus lacteus]]
[[Category: Single protein]]
[[Category: Ascenzi, P.]]
[[Category: Bolognesi, M.]]
[[Category: Deng, P.]]
[[Category: Dewilde, S.]]
[[Category: Geuens, E.]]
[[Category: Hale, A.]]
[[Category: Moens, L.]]
[[Category: Nardini, M.]]
[[Category: Nienhaus, G.U.]]
[[Category: Nienhaus, K.]]
[[Category: Olson, J.S.]]
[[Category: Pesce, A.]]
[[Category: Riggs, A.]]
[[Category: HEM]]
[[Category: OXY]]
[[Category: SO4]]
[[Category: nerve tissue mini-hemoglobin]]
[[Category: oxygen affinity of c.lacteus mini-hemoglobin]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:30:36 2007''