View source for 104d

[[Image:104d.jpg|left|200px]]<br /><applet load="104d" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="104d" />
'''DNA DUPLEXES FLANKED BY HYBRID DUPLEXES: THE SOLUTION STRUCTURE OF CHIMERIC JUNCTIONS IN'''<br />

==Overview==
Hybrid duplexes and chimeric duplexes containing hybrid segments linked to pure DNA (or pure RNA) segments are involved in transcription and replication, as well as reverse transcription. A complete understanding of the mechanism of these processes requires detailed information on such duplexes and the junctions between duplexes of differing structure. Using two-dimensional NMR, restrained molecular dynamics and mechanics, and back-calculation refinement against the nuclear Overhauser effect spectra at various mixing times, we have determined the solution structure of the chimeric duplex [r(cgcg)d-(TATACGCG)]2 containing a pure DNA segment in the center of a hybrid duplex. The solution structure differs from the previously determined X-ray structure of the analogous duplex [r(gcg)d(TATACGC)]2, which was found to be A-form throughout [Wang, A.H.-J., et al. (1982) Nature 299, 601-604]. The basic features of the solution structure are (a) the RNA residues are all A-form with C3'-endo sugar conformations, (b) the central DNA segment is B-form, (c) the transition from A-form RNA sugar conformations to B-form DNA sugar conformations involves only the dT5 base step, and (d) although the sugar conformations of the DNA residues A6-G12 are closer to B-form, the basic helical properties of the peripheral RNA.DNA hybrid segments are closer to typical A-form than to B-form.

==About this Structure==
104D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=104D OCA]. 

==Reference==
DNA duplexes flanked by hybrid duplexes: the solution structure of chimeric junctions in [r(cgcg)d(TATACGCG)]2., Zhu L, Salazar M, Reid BR, Biochemistry. 1995 Feb 21;34(7):2372-80. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7857947 7857947]
[[Category: Protein complex]]
[[Category: Reid, B R.]]
[[Category: Salazar, M.]]
[[Category: Zhu, L.]]
[[Category: dna]]
[[Category: duplex]]
[[Category: hybrid]]
[[Category: junction]]
[[Category: nmr]]
[[Category: rna]]

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