View source for 1gtk

[[Image:1gtk.gif|left|200px]]<br />
<applet load="1gtk" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1gtk, resolution 1.66&Aring;" />
'''TIME-RESOLVED AND STATIC-ENSEMBLE STRUCTURAL CHEMISTRY OF HYDROXYMETHYLBILANE SYNTHASE'''<br />

==Overview==
The enzyme hydroxymethylbilane synthase (HMBS, EC 4.3.1.8), 313 amino acid, residues and MW 34 kDa, also known as porphobilinogen deaminase (PBGD), catalyses the stepwise polymerization of four molecules of porphobilinogen, (PBG) to the linear tetrapyrrole 1-hydroxymethylbilane. Several, crystallographic structures of HMBS have been previously determined, most, recently including by time-resolved Laue protein crystallography of the, Lys59Gln mutant form with reaction initiation undertaken by use of a flow, cell carrying the substrate PBG. In this paper we review these structures, and add new molecular graphics representations and analyses. Moreover we, present a new structure refined at 1.66 A resolution using diffraction, data recorded at cryo-temperature (100 K) in an attempt at ... [[http://ispc.weizmann.ac.il/pmbin/getpm?12555854 (full description)]]

==About this Structure==
1GTK is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with DPM as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Transferred_entry:_2.5.1.61 Transferred entry: 2.5.1.61]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.1.8 4.3.1.8]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GTK OCA]]. 

==Reference==
Time-resolved and static-ensemble structural chemistry of hydroxymethylbilane synthase., Helliwell JR, Nieh YP, Habash J, Faulder PF, Raftery J, Cianci M, Wulff M, Hadener A, Faraday Discuss. 2003;122:131-44; discussion 171-90. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12555854 12555854]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Transferred entry: 2.5.1.61]]
[[Category: Faulder, P.F.]]
[[Category: Habash, J.]]
[[Category: Hadener, A.]]
[[Category: Helliwell, J.R.]]
[[Category: Nieh, Y.P.]]
[[Category: Raftery, J.]]
[[Category: Wulff, M.]]
[[Category: DPM]]
[[Category: all alpha/beta]]
[[Category: biosynthesis of linear tetrapyrrole]]
[[Category: lyase]]

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