View source for 1jjx

[[Image:1jjx.jpg|left|200px]]<br /><applet load="1jjx" size="350" color="white" frame="true" align="right" spinBox="true" 
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'''Solution Structure of Recombinant Human Brain-type Fatty acid Binding Protein'''<br />

==Overview==
Human brain-type fatty acid-binding protein (B-FABP) has been, recombinantly expressed in Escherichia coli both unlabelled and, 15N-enriched for structure investigation in solution using high-resolution, NMR spectroscopy. The sequential assignments of the 1H and 15N resonances, were achieved by applying multidimensional homo- and heteronuclear NMR, experiments. The ensemble of the 20 final energy-minimized structures, representing human B-FABP in solution, have been calculated based on a, total of 2490 meaningful distance constraints. The overall B-FABP, structure exhibits the typical backbone conformation described for other, members of the FABP family, consisting often antiparallel beta-strands, (betaA to betaJ) that form two almost orthogonal beta-sheets, a, helix-turn-helix motif that closes the beta-barrel on one side, and a, short N-terminal helical loop. A comparison with the crystal structure of, the same protein complexed with docosahexaenoic acid reveals only minor, differences in both secondary structure and overall topology. Moreover, the NMR data indicate a close structural relationship between human B-FABP, and heart-type FABP with respect to fatty acid binding inside the protein, cavity.

==Disease==
Known diseases associated with this structure: Infundibular hypoplasia and hypopituitarism OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=313430 313430]], Mental retardation, X-linked, with isolated growth hormone deficiency OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=313430 313430]]

==About this Structure==
1JJX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JJX OCA]. 

==Reference==
Solution structure of fatty acid-binding protein from human brain., Rademacher M, Zimmerman AW, Ruterjans H, Veerkamp JH, Lucke C, Mol Cell Biochem. 2002 Oct;239(1-2):61-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12479569 12479569]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Luecke, C.]]
[[Category: Rademacher, M.]]
[[Category: Rueterjans, H.]]
[[Category: Veerkamp, J.H.]]
[[Category: Zimmerman, A.W.]]
[[Category: 15n isotope enrichment]]
[[Category: beta barrel]]
[[Category: fatty acid carrier]]
[[Category: lipid binding protein]]
[[Category: nmr spectroscopy]]

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