View source for 2c96

[[Image:2c96.gif|left|200px]]<br />
<applet load="2c96" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="2c96, resolution 1.80&Aring;" />
'''STRUCTURAL BASIS OF THE NUCLEOTIDE DRIVEN CONFORMATIONAL CHANGES IN THE AAA DOMAIN OF TRANSCRIPTION ACTIVATOR PSPF'''<br />

==Overview==
Bacterial enhancer-binding proteins (EBP) activate transcription by, hydrolyzing ATP to restructure the sigma(54)-RNA polymerase-promoter, complex. We compare six high resolution structures (&lt;2.1 A) of the AAA(+), domain of EBP phage shock protein F (PspF) including apo, AMPPNP, Mg(2+)-ATP, and ADP forms. These structures permit a description of the, atomic details underpinning the origins of the conformational changes, occurring during ATP hydrolysis. Conserved regions of PspF's AAA(+) domain, respond distinctively to nucleotide binding and hydrolysis, suggesting, functional roles during the hydrolysis cycle, which completely agree with, those derived from activities of PspF mutated at these positions. We, propose a putative atomic switch that is responsible for coupling, structural ... [[http://ispc.weizmann.ac.il/pmbin/getpm?16430918 (full description)]]

==About this Structure==
2C96 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with ATP as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2C96 OCA]]. 

==Reference==
Structural basis of the nucleotide driven conformational changes in the AAA+ domain of transcription activator PspF., Rappas M, Schumacher J, Niwa H, Buck M, Zhang X, J Mol Biol. 2006 Mar 24;357(2):481-92. Epub 2006 Jan 13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16430918 16430918]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Buck, M.]]
[[Category: Niwa, H.]]
[[Category: Rappas, M.]]
[[Category: Schumacher, J.]]
[[Category: Zhang, X.]]
[[Category: ATP]]
[[Category: aaa domain]]
[[Category: activator]]
[[Category: atp-binding]]
[[Category: atpase]]
[[Category: bacterial sigma54 activator]]
[[Category: dna-binding]]
[[Category: nucleotide-binding]]
[[Category: sensory transduction]]
[[Category: transcription regulation]]
[[Category: two-component regulatory system]]

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